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Reconstructing Islet Function In Vitro

  • Domenico Bosco
  • Paolo Meda
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 426)

Abstract

Throughout the day, insulin secretion and biosynthesis result from the activity of numerous β-cells which function in coordination. Until recently, it has been implicitly assumed that all these cells are alike, i.e. that they secrete synchronously and at the same rate under various conditions. However, several lines of evidence now show that the actual situation is more complex. Thus, in situ, individual β-cells show variable amounts of secretory granules and rough endoplasmic reticulum[1], different thresholds for glucose-induced electrical activity[2,3] and a variable ability to biosynthesize insulin[4]. Furthermore, the β-cells located at the periphery of the islets differ from those located in the center of the microorgan with regard to nucleus size[5,6], incorporation of [3H]-thymidine[7], number of gap junctions[8], extent of dye coupling[9], electrical activity[10,11], and ability to secrete insulin[12]. In vitro studies have also revealed marked differences between individual β-cells with respect to insulin release[13–17], electrical activity[18] and Ca2+ changes during stimulation[19,20] as well as with respect to protein biosynthesis[4] and glucose-activated metabolic redox changes[21–23]. This wide heterogeneity, which is reviewed in the first part of this chapter, raises the question of how β-cells achieve the proper coordination which is necessary to ensure appropriate insulin biosynthesis and secretion under continously changing physiological conditions.

Keywords

Insulin Secretion Pancreatic Islet Insulin Release Plaque Assay Insulin Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Domenico Bosco
    • 1
  • Paolo Meda
    • 1
  1. 1.Department of MorphologyUniversity of Geneva, C.M.U.Switzerland

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