Advertisement

Physiological Behavior of Functional Subpopulations of Single Pancreatic β-Cells

  • Marcia Hiriart
  • Ma. Teresa Martinez
  • Ma. del Carmen Ramírez-Medeles
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 426)

Abstract

Single pancreatic β-cells are heterogeneous, the following are observations of β-cell variability: insulin content and biosynthesis of individual β-cells (Pipeleers, 1987; Schuit et al., 1988), glucose transport (Tominaga et al., 1986), electrical activity (Dean and Mattews, 1970; Ashcroft et al., 1987) and insulin secretion (Salomon and Meda, 1986; Hiriart and Matteson, 1988; Hiriart and Ramirez-Medeles, 1991; Van Schravendijk et al., 1992).

Keywords

Insulin Secretion Secretion Rate Plaque Area Insulin Secretion Rate Medium Secretion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ammon, H.P.T., Glocker, C., Waldner, R.G., and Wahl, M.A., 1989, Insulin release from pancreatic islets of fetal rats mediated by leucine b-BCH, tolbutamide, glibenclamide, arginine, potassium chloride, and theophylline does not require stimulation of Ca2+ net uptake, Cell Calcium 10:441–450.PubMedCrossRefGoogle Scholar
  2. Ashcroft, F.M., Harrison, D.E., Ashcroft, S.J.H., 1987, A potassium channel modulated by glucose metabolism in rat pancreatic B-cells, in “Biophysics of the Pancreatic B-Cells,” I. Atwater, E. Rojas, B. Soria, eds., Plenum Press, New York.Google Scholar
  3. Dean, P.M., and Mattews, E.K., 1970, Glucose-induced electrical activity in pancreatic islet cells, J. Physiol 210: 255–264.PubMedGoogle Scholar
  4. Hiriart, M. and Ramírez-Medeles, M.C., 1991, Functional subpopulations of individual pancreatic B-cells in culture, Endocrinology 128: 3193–3198.PubMedCrossRefGoogle Scholar
  5. Hiriart, M., and Ramírez-Medeles, M.C., 1993, Muscarinic modulation of insulin secretion by single pancreatic B-cells, Mol. and Cellular Endocrinology 93: 63–69.CrossRefGoogle Scholar
  6. Hiriart, M., and Matteson, D.R., 1988, Na channels and two types of Ca channels in rat pancreatic B cells identified with the reverse hemolytic plaque assay, J. Gen. Physiol. 91: 617–639.PubMedCrossRefGoogle Scholar
  7. Horta, J., Hiriart, M. and Cota, G., 1991, Differential expression of Na channels in functional subpopulations of rat lactotropes, Am. J. Physiol. 261: C865–C871.PubMedGoogle Scholar
  8. Neill, J.D., and Frawley, L. S., 1983, Detection of hormone release from individual cells in mixed populations using a reverse hemolytic plaque assay, Endocrinology 112: 1135–1137.PubMedCrossRefGoogle Scholar
  9. Pipeleers, D., 1987, The biosociology of pancreatic B cells, Diabetologia 30:277–291.PubMedCrossRefGoogle Scholar
  10. Salomon, D., and Meda, P., 1986, Heterogeneity and contact-dependent regulation of hormone secretion by individual B cells, Exp. Cell. Res. 162:507–520.PubMedCrossRefGoogle Scholar
  11. Schuit, F.C., In’tVeld, P.A., and Pipeleers, D.G., 1988, Glucose stimulates proinsulin biosynthesis by a dose-dependent recruitment of pancreatic beta cells, Proc. Natl. Acad. Sci. USA. 85: 3865–3869.PubMedCrossRefGoogle Scholar
  12. Tominaga, M., Komiya, I., Johnson, J.H., Inman, L., Alam, T., Moltz, J., Crider, B., Stefan, Y., Baetens, D., McCorkle, K., Orci, L., and Unger, R.H., 1986, Loss of insulin response to glucose but not arginine during the development of auto immune diabetes in BB/W rats: relationships to islet volume and glucose transport rate, Proc. Natl. Acad. Sci. USA. 83:9749–9753.PubMedCrossRefGoogle Scholar
  13. Van Schravendijk, C.F.H., Kiekens, R., and Pipeleers, D.G., 1992, Pancreatic β cell heterogeneity in glucose-induced insulin secretion, J. Biol. Chem. 267: 21344–21348.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Marcia Hiriart
    • 1
  • Ma. Teresa Martinez
    • 1
  • Ma. del Carmen Ramírez-Medeles
    • 2
  1. 1.Department of Bioenergetics, Instituto de Fisiología CelularUniversidad Nacional Autónoma de MéxicoMexico
  2. 2.Department of Biological SystemsUniversidad Autónoma MetropolitanaXochimilco, D.F.Mexico

Personalised recommendations