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Cell Culture Conditions Influence Glucose-Induced [Ca2+]i Responses in Isolated Rat Pancreatic B Cells

  • M.-H. Antoine
  • P. Lebrun
  • A. Herchuelz
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 426)

Abstract

An increase in the cytosolic free Ca2+ concentration ([Ca2+]i) in the pancreatic B cell is thought to represent a key event in the process of glucose-induced insulin release. The availability of fluorophores like Quin 2, Fura 2 and Indo 1 has recently allowed to demonstrate this increase both in intact pancreatic islets and in isolated B cells from different species. The pattern of [Ca2+]i changes found in intact islets in response to glucose appears consistent among different studies and consists in fast Ca2+ oscillations that parallel the oscillatory bursts of electrical activity. In intact B cells, different patterns of [Ca2+]i changes have been observed consisting either in discrete and regular spikes arising from a constant basal level (frequency: 0.2 to 0.5/min) [1] or in sinusodal oscillations [2,3] (frequency 2 to 3/min) observed on top of an elevated level of [Ca2+]i.

Keywords

Cell Culture Condition Calcium Oscillator Regular Spike Intact Islet Biphasic Increase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    B. Hellman, E. Gylfe, E. Grapengiesser, P.-E. Lund and A. Berts, Cytoplasmic Ca2+ oscillations in pancreatic B cells, Biochim. Biophys. Acta 1113:295 (1992).PubMedCrossRefGoogle Scholar
  2. 2.
    A. Herchuelz, R. Pochet, C. Pastiels and A. Van Praet, Heterogeneous changes in [Ca2+]i induced by glucose, tolbutamide and K+ in single rat pancreatic B cells, Cell Calcium 12:577 (1991).PubMedCrossRefGoogle Scholar
  3. 3.
    M.J. Berridge and A. Galione, Cytosolic calcium oscillators, FASEB J. 2:3074 (1988).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • M.-H. Antoine
    • 1
  • P. Lebrun
    • 1
  • A. Herchuelz
    • 1
  1. 1.Laboratory of PharmacologyBrussels University, School of MedicineBrusselsBelgium

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