Cell Culture Conditions Influence Glucose-Induced [Ca2+]i Responses in Isolated Rat Pancreatic B Cells
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An increase in the cytosolic free Ca2+ concentration ([Ca2+]i) in the pancreatic B cell is thought to represent a key event in the process of glucose-induced insulin release. The availability of fluorophores like Quin 2, Fura 2 and Indo 1 has recently allowed to demonstrate this increase both in intact pancreatic islets and in isolated B cells from different species. The pattern of [Ca2+]i changes found in intact islets in response to glucose appears consistent among different studies and consists in fast Ca2+ oscillations that parallel the oscillatory bursts of electrical activity. In intact B cells, different patterns of [Ca2+]i changes have been observed consisting either in discrete and regular spikes arising from a constant basal level (frequency: 0.2 to 0.5/min)  or in sinusodal oscillations [2,3] (frequency 2 to 3/min) observed on top of an elevated level of [Ca2+]i.
KeywordsCell Culture Condition Calcium Oscillator Regular Spike Intact Islet Biphasic Increase
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