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Phe—Met—Arg—Phe—NH2 (FMRFa)-Related Peptides Inhibit Na/Ca Exchange in Pancreatic B Cells

  • F. Van Eylen
  • P. Gourlet
  • A. Vandermeers
  • P. Lebrun
  • A. Herchuelz
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  • 118 Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 426)

Abstract

Two processes located at the cell plasma membrane appear to mediate Ca2+ extrusion from cells: the Ca2+ ATP ase and the process of Na/Ca exchange [1,2]. In the heart, Na/Ca exchange appears to be the predominant mechanism for Ca2+ extrusion, being able to restore and control basal Ca2+ level on a beat-to-beat basis [3]. Although the existence of a process of Na/Ca exchange in the pancreatic B cell has been postulated for many years, the process was only recently characterized and shown to regulate cytosolic free Ca2+ concentration within the physiological range. One major factor that has hindered rapid progresses in our knowledge of the process of Na/Ca exchange is the lack of specific inhibitors of the exchange. Recently, opioïd analogs (e.g. naloxone) were shown to inhibit Na/Ca exchange in cardiac sarcolemmal vesicles [4]. Likewise, peptides related to Phe— Met—Arg—Phe—NH2 (FMRFa) with naloxone-like activity were observed to induce complete inhibition of Na/Ca exchange [5]. In addition, it was suggested that endogenous analogs of FMRFa may exert some of their physiological modulatory role by inhibiting Na/Ca exchange [5]. FMRFa is a molluscan peptide with potent effects on heart, as well as on other muscles and nerves of molluscs. FMRFa-related peptides have also been localized in several mammalian tissues and appear to exert various actions in eluding antagonism to opioïd analgesia [6,7]. The aim of the present study was to examine the effects of FMRFa-related peptides on Na/Ca exchange in rat pancreatic islet cells.

Keywords

Related Peptide Cell Plasma Membrane Solid Phase Peptide Synthesis Basal Uptake Hydroxyamino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • F. Van Eylen
    • 1
  • P. Gourlet
    • 2
  • A. Vandermeers
    • 2
  • P. Lebrun
    • 1
  • A. Herchuelz
    • 1
  1. 1.Laboratory of PharmacologyBrussels Universit y School of MedicineBrusselsBelgium
  2. 2.Laboratory of Biochemistry and NutritionBrussels Universit y School of MedicineBrusselsBelgium

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