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Metabolism of Phosphatidylcholine in Mouse Pancreatic Islets

  • K. Capito
  • S. E. Hansen
  • C. J. Hedeskov
  • P. Thams
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  • 119 Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 426)

Abstract

Phosphatidylcholine (PC) is the most abundant phospholipid in pancreatic islets. “De novo” synthesis of islet PC has been shown to be enhanced by glucose1 and PC is degredated during glucose stimulation of insulin secretion2 thus implicating PC turnover as a regulatory event in glucose-induced insulin secretion. The aim of the present investigation was to evaluate the effect of insulin secretagogues on degradation of islet PC as measured by the formation of water soluble choline-containing metabolites of PC in mouse pancreatic islets.

Keywords

Pancreatic Islet Insulin Secretagogue Krebs Buffer Mouse Pancreatic Islet Choline Metabolite 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Hoffmann, J.M., and Laychock, S.G., 1988, Choline turnover in phosphatidylcholine of pancreatic islets, Diabetes 37:1489–1498.CrossRefGoogle Scholar
  2. 2.
    Turk, J., Wolf, B.A., Lefkowith, J.B., Stump, W.T. and McDaniel, M.L., 1986, Glucose-induced phospholipid hydrolysis in isolated pancreatic islets: quantitative effects on the phospholipid content of arachidonate and other fatty acids, Biochim. Biophys. Acta 879:399–409.PubMedCrossRefGoogle Scholar
  3. 3.
    Cook, S.J. and Wakelam, J.O., 1989, Analysis of the water-soluble products of phosphatidylcholine breakdown by ion-exchange chromatography, Biochem. J. 263:581–587.PubMedGoogle Scholar
  4. 4.
    Wolf, B.A., Easom, R.A., Hughes, J.H., McDaniel, M.L. and Turk, J., 1989, Secretagogue-induced diacyl-glycerol accumulation in isolated pancreatic islets. Mass spectrometric characterization of the fatty acyl content indicates multiple mechanisms of generation, Biochemistry 28:4291–4301.PubMedCrossRefGoogle Scholar
  5. 5.
    Tijburg, L.B.M., Geelen, M.J.H. and Von Golde, L.M.G., 1989, Regulation of the biosynthesis of triacyl-glycerol, phosphatidylcholine and phosphatidylethanolamine in the liver, Biochim. Biophys. Acta 1004:1–19.PubMedCrossRefGoogle Scholar
  6. 6.
    Dunlop, M. and Metz, S.A., 1989, A phospholipase D-like mechanism in pancreatic islet cells: stimulation by calcium ionophore, phorbol ester and sodium fluoride, Biochem. Biophys. Res. Commun. 163:922–928.PubMedCrossRefGoogle Scholar
  7. 7.
    Laychock, S.G., 1982, Phospholipase A2 activity in pancreatic islets is calcium-dependent and stimulated by glucose, Cell Calcium 3:43–54.PubMedCrossRefGoogle Scholar
  8. 8.
    Ramanadham, S., Gross, R.W., Han, X. and Turk, J., 1993, Inhbition of arachidonate release by secretagogue-stimulated pancreatic islets suppresses both insulin secretion and the rise in β-cell cytosolic calcium ion concentration, Biochemistry 32:337–346.PubMedCrossRefGoogle Scholar
  9. 9.
    Gross, R.W., Ramanadham, S., Kruszka, K.K., Han, X. and Turk, J., 1993, Rat and human pancreatic islet cells contain a calcium ion independent phospholipase A2 activity selective for hydrolysis of arachidonate which is stimulated by adenosine triphosphate and is specifically localized to islet β-cells, Biochemistry 32:327–336.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • K. Capito
    • 1
  • S. E. Hansen
    • 1
  • C. J. Hedeskov
    • 1
  • P. Thams
    • 1
  1. 1.Department of Medical Biochemistry and GeneticsThe Panum InstituteCopenhagen NDenmark

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