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Characterization of Purinergic Receptor-Evoked Increases in Intracellular Ca2+ Transients in Isolated Human and Rodent Insulin-Secreting Cells

Purinergic Receptor Signalling and [Ca2+]. in Human β-Cells
  • P. E. Squires
  • R. F. L. James
  • N. J. M. London
  • M. J. Dunne
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 426)

Abstract

In vivo many factors determine the regulation of insulin secretion from the β-cells of the pancreatic islets of Langerhans. The most predominant influence is a rise in the concentration of plasma glucose, which will promote secretion by mechanisms associated with Ca2+ influx across the plasma membrane(1). Insulin release is also governed by changes in the concentration of circulating amino acids, gastrointestinal hormones, neuropeptides and neurotransmitters. These influences are important avenues for β-cell regulation as they not only provide a link between the gastrointestinal tract and the pancreatic islets — the enteroinsular axis, but they also govern the neurohormonal control of secretion. For many years it has been recognised that extracellular ATP is a potent insulin secretagogue(2). In vitro it has been demonstrated using rodent and clonal insulin-secreting cells that purinergic receptor activation is coupled to an increase in [Ca2+]i(3–6). However, few studies have examined intracellular Ca2+ signalling in isolated human insulin-secreting cells(4,7–10). In a recent paper we have shown that ATP and other agonists of the purinergic receptor evoke marked increases in [Ca2+]i in β-cells isolated from transplantable human islets of Langerhans(11). In this paper we examine: (i) the purinergic receptor subtype present in human tissue, (ii) compare purinergic receptor-evoked increases in [Ca2+]i in both rodent and human β-cells and (iii) demonstrate that intracellular Ca2+ signals can also be recorded in cryopreserved human β-cells originally isolated from cadaver organ donors.

Keywords

RINm5F Cell United Kingdom Introduction Enteroinsular Axis Rodent Tissue Rodent Islet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • P. E. Squires
    • 1
  • R. F. L. James
    • 2
  • N. J. M. London
    • 2
  • M. J. Dunne
    • 1
  1. 1.Department of Biomedicai ScienceThe University of SheffieldSheffieldUK
  2. 2.Department of Surgery, School of MedicineThe University of LeicesterLeicesterUK

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