Trans-Acting Factor(s) Confer Glucose-Responsive Transcriptional Regulation in the Insulin Gene

  • Steve Goodison
  • Stephen J. H. Ashcroft
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 426)


In adult mammals the production of insulin is restricted to the β-cells of the islets of Langerhans. The β-cell specificity of insulin gene expression has been shown to be dependent on cis-acting sequences in the upstream non-coding region of the gene (1). Transfection studies have used mammalian insulin gene promoters to express reporter genes in a number of β-cell lines and have demonstrated that specific sequences exert positive or negative effects upon gene expression (2,3).


Reporter Gene Expression Insulin Gene Insulin Gene Expression John Radcliffe Hospital Express Reporter Gene 
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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Steve Goodison
    • 1
  • Stephen J. H. Ashcroft
    • 1
  1. 1.Nuffield Dept. of Clinical Biochemistry, John Radcliffe HospitalUniversity of OxfordHeadington, OxfordUK

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