Abstract
Arachidonate 12-lipoxygenase introduces one oxygen molecule regiospecifically and stereoselectively into the carbon 12 of arachidonic acid. Our group has demonstrated the occurrence of two isoforms of 12-lipoxygenase (platelet-type and leukocyte-type) which were distinguished in terms of substrate specificity, the cross-reactivity of their antibodies and the homology of their amino acid sequences.1,2 The leukocyte-type 12-lipoxy-genases have been detected not only in leukocytes, but also in several non-hematopoietic cell types, including bovine tracheas,3 porcine anterior pituitary glands,4 canine cerebra5 and rat pineal glands.6 The platelet-type 12-lipoxygenases were found in platelets of many animal species and also in human and mouse skin cells.7–9 For a better understanding of the physiological role of the platelet-type 12-lipoxygenase, we attempted to further investigate the expression and the distribution of the enzyme by immunohistochemical study. However, the purification of platelet-type 12-lipoxygenase as antigen was difficult, and no polyclonal antibody with a high affinity for platelet-type enzyme was available until recently. Therefore, in this study we attempted to purify a recombinant enzyme as an antigen.
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Nakamura, M., Ueda, N., Yamamoto, S., Ishimura, K., Uchida, N., Arase, S. (1997). Tissue Distribution and Subcellular Localization of Platelet-Type Arachidonate 12-Lipoxygenase. In: Honn, K.V., Marnett, L.J., Nigam, S., Jones, R.L., Wong, P.YK. (eds) Eicosanoids and other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury 3. Advances in Experimental Medicine and Biology, vol 407. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1813-0_3
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DOI: https://doi.org/10.1007/978-1-4899-1813-0_3
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