Abstract
It is well known that activation of thromboxane A2 (TP) receptors results in platelet aggregation, vasoconstriction, and mitogenesis of vascular smooth muscle cells1. These various effects of thromboxane A2 (TXA2) may be exerted through different TP receptor subtypes or a single receptor protein coupled to different intracellular signal transduction pathways. Southern blot analysis of genomic DNA showed that there is only a single copy gene for this receptor. To determine wether the bovine TP receptor differs from receptors identified in human placenta2, and human endothelium3, we examined the molecular structure of the bovine receptor. In additional experiments we investigated the tissue distribution of this novel TP receptor in bovine tissues and vascular smooth muscle cells (VSMC) by means of reverse transcriptase-polymerase chain reaction (RT-PCR). Recent studies from our laboratory have shown that TXA2 potentiates the mitogenic effects of thrombin on VSMC proliferation4. As a possible mechanism for these potentiating effects, we studied TP receptor regulation on mRNA-level.
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Muck, S., Schrör, K. (1997). Cloning, Tissue-Specific Expression and Regulation of the Bovine Thromboxane A2 Receptor. In: Sinzinger, H., Samuelsson, B., Vane, J.R., Paoletti, R., Ramwell, P., Wong, P.YK. (eds) Recent Advances in Prostaglandin, Thromboxane, and Leukotriene Research. Advances in Experimental Medicine and Biology, vol 433. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1810-9_8
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DOI: https://doi.org/10.1007/978-1-4899-1810-9_8
Publisher Name: Springer, Boston, MA
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