Abstract
8-iso-prostaglandin F2α (8-iso-PGF2α), an F2-isoprostane, is produced in vivo by a cyclooxygenase-independent, free radical-catalyzed mechanism involving peroxidation of arachidonic acid1. This isoprostane is a highly potent renal vasoconstrictor2 and stimulates proliferation of cultured rat aortic smooth muscle cells (AoSMC) through enhancement of phosphoinositide turnover3. AoSMC have distinct F2-isoprostane binding sites, although these putative receptors bear homology to the thromboxane A2 (TxA2) receptor4. In this study, we report the activation of mitogen activated protein kinases (MAP-kinases)5 by 8-iso-PGF2α in AoSMC and its regulating mechanism involving protein kinase C (PKC) and pertussis toxin (PT)-sensitive GTP binding proteins.
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References
J.D. Morrow, K.E Hill, R.F. Burk, T.M. Nammour, K.F. Badr, and L.J. Roberts II. A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cycloaxygenase, free radical-catalyzed mechanism. Proc. Natl. Acad. Sci. USA 87: 9383 (1992).
K. Takahashi, T.M. Nammour, M. Fukunaga, J. Ebert, J.D. Morrow, L.J. Robots II, R.L. Hoover, and K.F. Badr. Glomerular actions of a free radical-generated novel prostaglandin, 8-epi-prostaglandin F2α, in the rat: Evidence for interaction with thromboxane A2 receptors. J. Clin. Invest. 90: 136 (1992).
M. Fukunaga, N. Makita, L.J. Roberts II, J.D. Morrow, K. Takahashi, and K.F. Badr. Evidence for the existence of F2-isoprostane receptors on rat vascular smooth muscle cells. Am. J. Physiol. (Cell Physiol. 33) 264: C1619 (1993).
T. Yura, M. Fukunaga, R. Grygorczyk, N. Makita, K. Takahashi, and K.F. Badr. Molecular and functional evidence for the distinct nature of F2-isoprostane receptors from those of thromboxane A2. Adv. Prostagl. Thrombox. Leukotr. Res. 23: 237 (1995).
R. Seger, and E.G. Krebs. The MAPK signalingcascade. FASEB J. 9: 726 (1995).
M.J. Berridge, and R.F. Irvine. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature Lond. 1984: 312 (1984).
J.D. Morrow, K.P. Moore, J.A. Awad, M.D. Ravenscraft, G. Marini, K.F. Badr, R. Williams, and L.J. Roberts II. Marked overproduction of non-cyclooxygenase derived prostanoids (F2-isoprostanes) in the hepatorenal syndrome. J. Lipid Mediators 6: 417 (1993).
J.D. Morrow, B. Frei, A.W. Longmire, J.M. Gaziano, S.M. Lynch, Y. Shyr, W.E Strauss, J.A. Oates, L.J. Roberts II. Increase in circulating products of lipid peroxidation (F2-isoprostane) in smokers.: Smoking as a cause of oxidative damage. N. Engl J. Med. 332: 1198 (1995).
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Fukunaga, M., Yura, T., Takahashi, K., Badr, K.F. (1997). Regulation of MAP-Kinase Activation by 8-Iso-Prostaglandin F2a in Cultured Rat Aortic Smooth Muscle Cells. In: Sinzinger, H., Samuelsson, B., Vane, J.R., Paoletti, R., Ramwell, P., Wong, P.YK. (eds) Recent Advances in Prostaglandin, Thromboxane, and Leukotriene Research. Advances in Experimental Medicine and Biology, vol 433. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1810-9_40
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DOI: https://doi.org/10.1007/978-1-4899-1810-9_40
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