Abstract
In 1976, Vane et al discovered prostacyclin (PGI2) that is a potent inhibitor of platelet secretion and aggregation, smooth muscle cell proliferation and vasoconstriction1–3. It is considered to play a key role in vasoprotection and its deficiency may lead to thrombosis and other vascular lesions4, 5. Biosynthesis of PGI2 is catalyzed serially by phospholipase A2 which liberates arachidonic acid from the sn-2 position of membrane phospholipids, prostaglandin H2 synthase, a bifunctional enzyme which catalyzes the conversion of arachidonic acid to prostaglandin G2 and subsequently to prostaglandin H2, and prostacyclin synthase (PGIS, EC 5.3.99.4) which catalyzes the formation of PGI2 from prostaglandin H2. PGIS has been shown to be widely distributed, predominantly in vascular endothelial and smooth muscle cells6, 7. The enzyme is a membrane-bound protein located in the endoplasmic reticulum. It exhibits spectral characteristics of cytochrome P450s but has no mono-oxygenase activity8, 9. Instead, it catalyzes an isomerization reaction and thus does not require a reductase to initiate the catalytic activity. A description has been given of the cDNA of human PGIS10 and the deduced amino acid sequence. In the present study, the genomic organization of the human PGIS gene was investigated11, and the association study of essential hypertension was carried out using a novel CA/TG dinucleotide repeat polymorphism in this gene12.
Keywords
- Essential Hypertension
- Genomic Clone
- Smooth Muscle Cell Proliferation
- Repeat Allele
- Dinucleotide Repeat Polymorphism
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
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Nakayama, T., Soma, M., Kanmatsuse, K. (1997). Organization of the Human Prostacyclin Synthase Gene and Association Analysis of a Novel CA Repeat in Essential Hypertension. In: Sinzinger, H., Samuelsson, B., Vane, J.R., Paoletti, R., Ramwell, P., Wong, P.YK. (eds) Recent Advances in Prostaglandin, Thromboxane, and Leukotriene Research. Advances in Experimental Medicine and Biology, vol 433. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1810-9_26
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DOI: https://doi.org/10.1007/978-1-4899-1810-9_26
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