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Immunoaffinity Purification of Membrane Fractions from Mammalian Cells

  • Peter J. Richardson
  • J. Paul Luzio
Part of the Subcellular Biochemistry book series (SCBI, volume 12)

Abstract

A wide variety of techniques have been developed for the subfractionation of mammalian cell membranes and organelles. The most widely used are density gradient centrifugation, free-flow electrophoresis, polymer phase partition, fluorescent activated “cell-sorting,” and immunoaffinity purification. While the immunoaffinity approach relies totally on antibody-antigen reactions, the specificity of the other systems, which depend largely on physical differences between particles, can be increased by the selective use of affinity procedures. This chapter, after discussing the relative merits of other membrane separation techniques, is concerned primarily with the design, application, and advantages of immunoaffinity purification using a solid matrix.

Keywords

Solid Support Affinity Purification Coated Vesicle Ehrlich Ascites Carcinoma Cell Plasma Membrane Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Peter J. Richardson
    • 1
  • J. Paul Luzio
    • 1
  1. 1.Department of Clinical BiochemistryUniversity of CambridgeCambridgeUK

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