Biochemistry and Pathophysiology of the Molecular Forms of Cholinesterases

  • Zoltán Rakonczay
  • Stephen Brimijoin
Part of the Subcellular Biochemistry book series (SCBI, volume 12)


Acetylcholinesterase (acetylcholine acetylhydrolase, AChE: EC and butyrylcholinesterase (acylcholine acylhydrolase, BuChE: EC both possess the capacity to hydrolyze choline esters, although the latter accepts a much wider variety of substrates. These two enzymes are found in a large number of excitable and nonexcitable tissues in most species, including humans (for review see Silver, 1974). The two types of ChE (generic abbreviation for any Cholinesterase) are readily distinguished not only by their substrate specificity but also by their response to selective inhibitors.


Amyotrophic Lateral Sclerosis Amniotic Fluid AChE Activity Molecular Form Extensor Digitorum Longus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used in this chapter




Alzheimer’s disease


amyotrophic lateral sclerosis




complementary DNA


choline acetyltransferase




cerebrospinal fluid


extensor digitorum longus


fluorescence-activated cell sorter


monoclonal antibody


muscular dysgenesis


motor endplate disease


neural tube defects


paroxysmal nocturnal hemoglobinuria




red blood cell


superior cervical ganglion.


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  1. Abe, T., Sakai, M., and Saisu, H., 1983, A monoclonal antibody against catalytic subunits of acetylcholinesterase in the electric organ of an electric ray, Narke japonica. Neurosci. Lett. 38: 61–66.Google Scholar
  2. Adams, C. W., Marples, E. A., and Trounce, J. R., 1960, Achalasia of cardia and Hirschsprung’s disease: The amount and distribution of cholinesterases. Clin. Sci. 19: 473–481.PubMedGoogle Scholar
  3. Adamson, E. D., 1977, Acetylcholinesterase in mouse brain, erythrocytes and muscle. J. Neurochem. 28: 605–615PubMedGoogle Scholar
  4. Adamson, E. C., Ayers, S. E., Deussen, Z. A., and Graham, C. F., 1975, Analysis of the forms of acetylcholinesterase from adult mouse brain. Biochem. J. 147: 205–214.PubMedGoogle Scholar
  5. Anglister, L., Tarrab-Hazdai, R., Fuchs, S., and Silman, I., 1979, Immunological cross-reactivity between electric eel acetylcholinesterase and rat tail-tendon collagen. Eur. J. Biochem. 94: 25–29.PubMedGoogle Scholar
  6. Arendt, T., Bigl, V., Tennstedt, A., and Arendt, A., 1985, Neuronal loss in different parts of the nucleus basalis is related to neuritic plaque formation in cortical target areas in Alzheimer’s disease. Neuroscience 14: 1–14.PubMedGoogle Scholar
  7. Arendt, T., Bigl, V., Walther, F., and Sonntag, M., 1984, Decreased ratio of CSF acetylcholinesterase to butyrylcholinesterase activity in Alzheimer’s disease. Lancet i: 173.Google Scholar
  8. Atack, J. R., Perry, E. K., Bonham, J. R., Perry, R. H., Tomlinson, B. E., Blessed, G., and Fairbairn, A., 1983, Molecular forms of acetylcholinesterase in senile dementia of Alzheimer type: Selective loss of the intermediate (10S) form. Neurosci. Lett. 40: 199–204.PubMedGoogle Scholar
  9. Atack, J. R., Perry, E. K., Perry, R. H., Wilson, I. D., Bober, M. J., Blessed, G., and Tomlinson, B. E., 1985, Blood acetyl- and butyrylcholinesterases in senile dementia of Alzheimer type. J. Neurol. Sci. 70: 1–12.PubMedGoogle Scholar
  10. Atack, J. R., Perry, E. K., Bonham, J. R., Candy, J. M., and Perry, R. H., 1986, Molecular forms of acetylcholinesterase and butyrylcholinesterase in the aged human central nervous system. J. Neurochem. 47: 263–277.PubMedGoogle Scholar
  11. Auditore, J. V., and Hartmann, R. C., 1959, Paroxysmal nocturnal hemoglobinuria. II. Erythrocyte acetylcholinesterase defect. Am. J. Med. 22: 401–410.Google Scholar
  12. Barth, F., and Ghandour, M. S., 1983, Cellular localization of butyrylcholinesterase in adult rat cerebellum determined by immunofluorescence. Neurosci. Lett. 39: 149–153.PubMedGoogle Scholar
  13. Benda, P., Tsuji, S., Daussant, J., and Changeux, J.-P., 1970, Localization of acetylcholinesterase by immunofluorescence in eel electroplax. Nature 225: 1149–1150.PubMedGoogle Scholar
  14. Bjornskov, E. K., Dekker, N. D., Norris, F. H., and Stuart, M. E., 1975, Endplate morphology in amyotrophic lateral sclerosis. Arch. Neurol. 32: 711–712.PubMedGoogle Scholar
  15. Bleisch, W. V., Luine, V. N., and McEwen, B. S., 1983, Molecular forms of acetylcholinesterase: Regulation in a testosterone-sensitive nerve-muscle axis. J. Neurochem. 40: 1523–1528.PubMedGoogle Scholar
  16. Bon, S., and Massoulié, J., 1980, Collagen-tailed and hydrophobic components of acetylcholinesterase in Torpedo marmorata electric organ. Biochemistry 77: 4464–4468.Google Scholar
  17. Bon, S., Cartaud, J., and Massoulié, J., 1978, The dependence of acetylcholinesterase aggregation at low ionic strength upon a polyanionic component. Eur. J. Biochem. 85: 1–14.PubMedGoogle Scholar
  18. Bon, S., Vigny, M., and Massoulié, J., 1979, Asymmetric and globular forms of acetylcholinesterase in mammals and birds. Proc. Natl. Acad. Sci. U.S.A. 76: 2546–2550.PubMedGoogle Scholar
  19. Bonham, J. R., and Atack, J. R., 1983, A neural tube defect specific form of acetylcholinesterase in amniotic fluid. Clin. Chim. Acta 135: 233–237.PubMedGoogle Scholar
  20. Bonham, J. R., Dale, G., Scott, D., and Wagget, J., 1985, Molecular forms of acetylcholinesterase in Hirschsprung’s disease. Clin. Chim. Acta 145: 297–305.PubMedGoogle Scholar
  21. Bowden-Essien, F., 1972, An in vitro study of normal and mutant myogenesis in the mouse. Dev. Biol. 27: 351–364.PubMedGoogle Scholar
  22. Bowen, D. M., Smith, C. B., White, P., and Davison, A. N., 1976, Neurotransmitter-related enzymes and indices of hypoxia in senile dementia and other abiotrophies. Brain 99: 459–496.PubMedGoogle Scholar
  23. Brandan, E., and Inestrosa, N. C., 1984, Binding of the asymmetric forms of acetylcholinesterase to heparin. Biochem. J. 221: 415–422.PubMedGoogle Scholar
  24. Brandan, E., Maldonado, M., Garrido, J., and Inestrosa, N. C., 1985, Anchorage of collagen-tailed acetylcholinesterase to the extracellular matrix is mediated by heparin sulfate proteoglycans. J. Cell Biol. 101: 985–992.PubMedGoogle Scholar
  25. Brimijoin, S., 1979, Axonal transport and subcellular distribution of molecular forms of acetylcholinesterase in rabbit sciatic nerve. Mol. Pharmacol. 15: 641–648.PubMedGoogle Scholar
  26. Brimijoin, S., 1983, Molecular forms of acetylcholinesterase in brain, nerve and muscle: Nature, localization and dynamics. Prog. Neurobiol. 21: 291–322.PubMedGoogle Scholar
  27. Brimijoin, S., and Mintz, K. P., 1985, Human acetylcholinesterase: Immunochemical studies with monoclonal antibodies. Biochim. Biophys. Acta 828: 290–297.PubMedGoogle Scholar
  28. Brimijoin, S., and Rakonczay, Z., 1986, Immunology and molecular biology of the cholinesterases: Current results and prospects. Int. Rev. Neurobiol. 28: 363–410.PubMedGoogle Scholar
  29. Brimijoin, S., and Schreiber, P. A., 1982, Reduced axonal transport of 10S acetylcholinesterase in dystrophic mice. Muscle Nerve 5: 405–410.PubMedGoogle Scholar
  30. Brimijoin, S., Mintz, K. P., and Alley, M. C., 1983a, Production and characterization of separate monoclonal antibodies to human acetylcholinesterase and butyrylcholinesterase. Mol. Pharmacol. 24: 513–520.PubMedGoogle Scholar
  31. Brimijoin, S., Hammond, P. I., and Petitt, R. M., 1986, Paroxysmal nocturnal hemoglobinuria: Erythrocyte acetylcholinesterase deficit analyzed by immunoassay and fluorescence-activated sorting. Mayo Clin. Proc. 61: 522–529.PubMedGoogle Scholar
  32. Brimijoin, S., Rakonczay, Z., and Hammond, P. I., 1987, Immunoassay of acetylcholinesterase. Fed. Proc. 46: 2557–2562.PubMedGoogle Scholar
  33. Brock, D. J. H., and Hayward, C., 1980, Gel electrophoresis of amniotic fluid acetylcholinesterase as an aid to the prenatal diagnosis of fetal defects. Clin. Chim. Acta 108: 135–141.PubMedGoogle Scholar
  34. Brock, D. J. H., Barron, L., and Heyningen, V., 1985, Prenatal diagnosis of neural-tube defects with a monoclonal antibody specific for acetylcholinesterase. Lancet i: 5–8.Google Scholar
  35. Brown, S. S., Kalow, W., Pilz, W., Whittaker, M., and Woronick, C. L., 1981, The plasma cholinesterases: A new perspective. Adv. Clin. Chem. 22: 1–123.PubMedGoogle Scholar
  36. Brzin, M., Sketelj, J., and Klinar, B., 1983, Cholinesterases. In Handbook of Neurochemistry (A. Lajtha, ed.), Vol. 4, pp. 251–292, Plenum Press, New York.Google Scholar
  37. Buamah, P. K., Evans, L., and Ward, M., 1980, Amniotic fluid acetylcholinesterase isoenzyme patterns in the diagnosis of neural tube defects. Clin. Chim. Acta 102: 147–151.PubMedGoogle Scholar
  38. Cardoso de Almeida, M. L., and Turner, M. J., 1983, The membrane form of variant surface glycoproteins of Trypanosoma brucei. Nature 302: 349–352.Google Scholar
  39. Carson, S., Bon, S., Vigny, M., Massoulié, J., and Fardeau, M., 1979, Distribution of acetylcholinesterase molecular forms in neural and non-neural sections of human muscle. FEBS Lett. 97: 348–352.PubMedGoogle Scholar
  40. Chan, S. L., Shirachi, D. Y., Bhargava, H. N., Gardner, E., and Trevor, A. J., 1972, Purification and properties of multiple forms of brain acetylcholinesterase (EC J. Neurochem. 19: 2747–2758.PubMedGoogle Scholar
  41. Chang, C.-H., and Blume, A. J., 1976, Heterogeneity of acetylcholinesterase in neuroblastoma. J. Neurochem. 27: 1427–1435.PubMedGoogle Scholar
  42. Chatonnet, A., and Bacou, F., 1983, Acetylcholinesterase molecular forms in the fast and slow muscles of the chicken and the pigeon. FEBS Lett. 161: 122–126.PubMedGoogle Scholar
  43. Chow, F.-L., Telen, M. J., and Rosse, W. F., 1985, The acetylcholinesterase deficit in paroxysmal nocturnal hemoglobinuria: Evidence that the enzyme is absent from the cell membrane. Blood 66: 940–945.PubMedGoogle Scholar
  44. Chubb, I. W., Pilowski, P. M., Springel, H. J., and Pollard, A. C., 1979, Acetylcholinesterase in amniotic fluid: An index of fetal neural development. Lancet i:688–690.Google Scholar
  45. Couraud, J. Y., Nicolet, M., and Hässig, R., 1985, Rapid axonal transport of three molecular forms of acetylcholinesterase in the frog sciatic nerve. Neuroscience 14: 1141–1147.PubMedGoogle Scholar
  46. Coyle, J. T., Price, D. L., and DeLong, M. R., 1983, Alzheimer’s disease: A disorder of cortical cholinergic innervation. Science 219: 1184–1190.PubMedGoogle Scholar
  47. Dacie, J. V., 1967, Paroxysmal nocturnal haemoglobinuria. In The Haemolytic Anaemias, Congential and Acquired. IV. Drug-Induced Haemolytic Anemia, Paroxysmal Haemoglobinuria (J. V. Dacie, ed.), pp. 1128–1160, Grune & Stratton, New York.Google Scholar
  48. Davies, P., 1979, Neurotransmitter-related enzymes in senile dementia of the Alzheimer type. Brain Res. 171: 319–327.PubMedGoogle Scholar
  49. Davies, P., and Maloney, A. J. F., 1976, Selective loss of central cholinergic neurons in Alzheimer’s disease. Lancet ii: 1403.Google Scholar
  50. Davies, P., and Verth, A. H., 1977, Regional distribution of the muscarinic receptor in normal and Alzheimer’s type dementia brain. Brain Res. 138: 385–392.PubMedGoogle Scholar
  51. Davies, R. O., Marton, A. V., and Kalow, W., 1960, The action of normal and atypical Cholinesterase of human serum upon a series of esters of choline. Can. J. Biochem. Physiol. 38: 545–551.PubMedGoogle Scholar
  52. Di Giamberardino, L., and Couraud, J. Y., 1978, Rapid accumulation of high molecular weight acetylcholinesterase in transected sciatic nerve. Nature 271: 170–172.PubMedGoogle Scholar
  53. Dockter, M. E., and Morrison, M., 1986, Paroxysmal nocturnal hemoglobinuria erythrocytes are of two distinct types: Positive or negative for acetylcholinesterase. Blood 67: 540–543.PubMedGoogle Scholar
  54. Doctor, B. P., Camp, S., Gentry, M. K., Taylor, S. S., and Taylor, P., 1983, Antigenic and structural differences in the catalytic subunits of the molecular forms of acetylcholinesterase. Proc. Natl. Acad. Sci. U.S.A. 80: 5767–5771.PubMedGoogle Scholar
  55. Duchen, L. W., Searle, A. G., and Strich, S. J., 1967, An hereditary motor end-plate disease in the mouse. J. Physiol. (Lond.) 189: 1–6P.Google Scholar
  56. Dudai, Y., Herzberg, M., and Silman, I., 1973, Molecular structures of acetylcholinesterase from electric organ tissue of the electric eel. Proc. Natl. Acad. Sci. U.S.A. 70: 2473–2476.PubMedGoogle Scholar
  57. Dutta-Choudhury, T. A., and Rosenberry, T. L., 1984, Human erythrocyte acetylcholinesterase is an amphipathic protein whose short membrane-binding domain is removed by papain digestion. J. Biol. Chem. 259: 5653–5660.PubMedGoogle Scholar
  58. Eckerson, H. W., Oseroff, A., Lockridge, O., and La Du, B. N., 1983, Immunological comparison of the usual and atypical human serum Cholinesterase phenotypes. Biochem. Genet. 21: 93–108.PubMedGoogle Scholar
  59. Elejalde, B. R., Peck, G., and De Elejalde, M. M., 1986, Determination of Cholinesterase and acetylcholinesterase in amniotic fluid. Clin. Genet. 29: 196–203.PubMedGoogle Scholar
  60. Ellman, G. L., Courtney, D. K., Andres, V., and Featherstone, R. M., 1961, A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7: 88–95.PubMedGoogle Scholar
  61. Fambrough, D. M., Engel, A. G., and Rosenberry, T. L., 1982, Acetylcholinesterase of human erythrocytes and neuromuscular junctions: Homologies revealed by monoclonal antibodies. Proc. Natl. Acad. Sci. U.S.A. 79: 1078–1082.PubMedGoogle Scholar
  62. Ferguson, M. A. J., Haldar, K., and Cross, G. A. M., 1985, Trypanosoma brucei variant surface glycoprotein has a sn-l, 2-dimyristyl glycerol membrane anchor at its COOH terminus. J. Biol. Chem. 260: 4963–4968.PubMedGoogle Scholar
  63. Fernandez, H. L., Duell, M. J., and Festoff, B. W., 1979a, Neurotrophic control of 16S acetylcholinesterase at the vertebrate neuromuscular junction. J. Neurobiol. 10: 441–454.PubMedGoogle Scholar
  64. Fernandez, H. L., Duell, M. J., and Festoff, B. W., 1979b, Cellular distribution of 16S acetylcholinesterase. J. Neurochem. 32: 581–585.PubMedGoogle Scholar
  65. Fernandez, H. L., Stiles, J. R., and Donoso, J. A., 1986, Skeletal muscle acetylcholinesterase molecular forms in amyotrophic lateral sclerosis. Muscle Nerve 9: 399–406.PubMedGoogle Scholar
  66. Festoff, B. W., and Fernandez, H. L., 1981, Plasma and red blood cell acetylcholinesterase in amyotrophic lateral sclerosis. Muscle Nerve 4: 41–47.PubMedGoogle Scholar
  67. Fishman, E. B., Siek, G. C., MacCallum, R. D., Bird, E. D., Volicer, L., and Marquis, J. K., 1986, Distribution of the molecular forms of acetylcholinesterase in human brain: Alterations in dementia of the Alzheimer type. Ann. Neurol. 19: 246–252.PubMedGoogle Scholar
  68. Futerman, A. H., Low, M. G., Ackermann, K. E., Sherman, W. R., and Silman, I., 1985a, Identification of covalently bound inositol in the hydrophobic membrane-anchoring domain of Torpedo acetylcholinesterase. Biochem. Biophys. Res. Commun. 129: 312–317.PubMedGoogle Scholar
  69. Futerman, A. H., Fiorini, R.-M., Roth, E., Low, M. G., and Silman, I., 1985b, Physicochemical behavior and structural characteristics of membrane-bound acetylcholinesterase from Torpedo electric organ. Effect of phosphatidylinositol-specific phospholipase C. Biochem. J. 226: 369–377.PubMedGoogle Scholar
  70. Gennari, K., and Brodbeck, U., 1985, Molecular forms of acetylcholinesterase from human caudate nucleus: Comparison of salt-soluble and detergent-soluble tetrameric enzyme species. J. Neurochem. 44: 697–704.PubMedGoogle Scholar
  71. Gisiger, V., and Stephens, H., 1982, Molecular forms and cytochemical distribution of acetylcholinesterase (AChE) in skeletal muscles of the 129/ReJ dystrophic mouse. Can. J. Neurol. Sci. 9: 361.Google Scholar
  72. Gisiger, V., and Stephens, H. R., 1983, Asymmetric and globular forms of AChE in slow and fast muscles of 129/ReJ normal and dystrophic mice. J. Neurochem. 41: 919–929.PubMedGoogle Scholar
  73. Gisiger, V., Vigny, M., Gautron, J., and Rieger, F., 1978, Acetylcholinesterase of rat sympathetic ganglion: Molecular forms, localization and effects of denervation. J. Neurochem. 30: 501–516.PubMedGoogle Scholar
  74. Goedde, H. W., Doenicke, A., and Altland, K., 1967, Pseudocholinesterasen. Pharmakogenetik, Biochemie, Klinik, Springer, Berlin.Google Scholar
  75. Gómez-Barriocanal, J., Barat, A., Escudero, E., Rodriguez-Borrajo, C., and Ramirez, G., 1981, Solubilization of collagen-tailed acetylcholinesterase from chick retina: Effect of different extraction procedures. J. Neurochem. 37: 1239–1249.PubMedGoogle Scholar
  76. Grassi, J., Vigny, M., and Massoulié, J., 1982, Molecular forms of acetylcholinesterase in bovine caudate nucleus and superior cervical ganglion: Solubility properties and hydrophobic character. J. Neurochem. 38: 457–469.PubMedGoogle Scholar
  77. Grassi, J., Massoulié, J., and Timpl, R., 1983, Relationship of collagen-tailed acetylcholinesterase with basal lamina components. Absence of binding with laminin, fibronectin, and collagen types IV and V, and lack of reactivity with different anti-collagen sera. Eur. J. Biochem. 133: 31–38.PubMedGoogle Scholar
  78. Greenberg, A. J., Parker, K. K., and Trevor, A. J., 1977, Immunochemical studies of mammalian brain acetylcholinesterase. J. Neurochem. 29: 911–917.PubMedGoogle Scholar
  79. Grosswald, D. E., and Dettbarn, W.-D., 1983, Nerve crush induced changes in molecular forms of acetylcholinesterase in soleus and extensor digitorum muscles. Exp. Neurol. 79: 519–531.Google Scholar
  80. Gurari, D., Silman, I., and Fuchs, S., 1974, Immunochemical and enzymatic properties of the electric eel acetylcholinesterase-antiacetylcholinesterase system. Eur. J. Biochem. 43: 179–187.PubMedGoogle Scholar
  81. Haas, R., Brandt, P. T., Knight, J., and Rosenberry, T. L., 1986, Identification of amine components in a glycolipid membrane-binding domain at the C-terminus of human erythrocyte acetylcholinesterase. Biochemistry 25: 3098–3105.PubMedGoogle Scholar
  82. Hall, Z. W., 1973, Multiple forms of acetylcholinesterase and their distribution in endplate and non-endplate regions of rat diaphragm muscle. J. Neurobiol. 4: 343–361.PubMedGoogle Scholar
  83. Hangaard, J., Sørensen, K., Brodbeck, U., and Nørgaard-Pedersen, B., 1984, Quantitative determination of acetylcholinesterase by enzyme antigen immunoassay: Methodological aspects and clinical use. Scand. J. Clin. Lab. Invest. 44: 717–724.PubMedGoogle Scholar
  84. Henderson, N. S., Tweedle, C. D., and Kabara, J. J., 1980, Cholinesterase in muscle of dystrophic hamsters (Bio-40.54). Neurochem. Res. 5: 1221–1230.PubMedGoogle Scholar
  85. Hedreen, J. C., Uhl, G. R., Bacon, S. J., Fambrough, D. M., and Price, D. L., 1984, Acetylcholin-esterase-immunoreactive axonal network in monkey visual cortex. J. Comp. Neurol. 226: 246–254.PubMedGoogle Scholar
  86. Hirano, A., 1973, Progress in the pathology of motor neuron diseases. Prog. Neuropathol. 2: 181–215.Google Scholar
  87. Hodkin, W. E., Giblett, E. R., Levine, H., Bauer, W., and Matulsky, A. G., 1965, Complete pseudocholinesterase deficiency: Genetic and immunologic characterization. J. Clin. Invest. 44: 486–493.Google Scholar
  88. Holzgreve, W., Rempen, A., and Beller, F. K., 1986, Fetal lymphangiectasia—another cause for a positive amniotic fluid acetylcholinesterase test. Arch. Gynecol. 239: 123–125.PubMedGoogle Scholar
  89. Hooper, M. W., and Vogel, F. S., 1976, The limbic system in Alzheimer’s disease. A neuropathologic investigation. Am. J. Pathol. 85: 1–20.Google Scholar
  90. Hsu, S.-M., Raine, L., and Fanger, H., 1981, Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29: 577–580.PubMedGoogle Scholar
  91. Igisu, H., Mawatari, S., Kuroiwa, Y., and Miyoshino, S., 1980, Erythrocyte acetylcholinesterase in Duchenne muscular dystrophy. Clin. Chim. Acta 105: 241–247.PubMedGoogle Scholar
  92. Inestrosa, N. C., Reiness, C. G., Reichardt, L. F., and Hall, Z. W., 1981, Cellular localization of the molecular forms of acetylcholinesterase in rat pheochromocytoma PC 12 cells treated with nerve growth factor. J. Neurosci. 1: 1260–1267.PubMedGoogle Scholar
  93. Jansz, H. S., and Cohen, J. A., 1962, Pseudocholinesterase from horse serum. I. Purification and properties of the enzyme. Biochim. Biophys. Acta 56: 531–537.PubMedGoogle Scholar
  94. Johnson, C. D., and Russell, R. L., 1975, A rapid, simple radiometric assay for Cholinesterase suitable for multiple determinations. Anal. Biochem. 64: 229–238.PubMedGoogle Scholar
  95. Kalderon, N., Silman, I., Blumberg, S., and Dudai, Y., 1970, A method for the purification of acetylcholinesterase by affinity chromatography. Biochim. Biophys. Acta 207: 560–562.PubMedGoogle Scholar
  96. Kalow, W., and Genest, K., 1957, A method for the detection of atypical forms of human serum Cholinesterase. Determination of dibucaine numbers. Can. J. Biochem. Physiol. 35: 339–346.PubMedGoogle Scholar
  97. Kamijo, K., Hiatt, R. B., and Koelle, G., 1953, Congenital megacolon. A comparison of the spastic and hypertrophied segments with respect to Cholinesterase activities and sensitivities to acetylcholines, DFP and the barium ion. Gastroenterology 24: 173–185.PubMedGoogle Scholar
  98. Karnovsky, M. J., and Roots, L., 1964, A “direct-coloring” thiocholine method for cholinesterases. J. Histochem. Cytochem. 12: 219–221.PubMedGoogle Scholar
  99. Kása, P., and Rakonczay, Z., 1982a, Histochemical and biochemical demonstration of the molecular forms of acetylcholinesterase in peripheral nerve of rat. Acta Histochem. 70: 244–257.PubMedGoogle Scholar
  100. Kása, P., and Rakonczay, Z., 1982b, Biochemical and histochemical evidence of 16S acetylcholinesterase in salivary glands. J. Neurochem. 38: 278–280.PubMedGoogle Scholar
  101. Koelle, G. B., and Friedenwald, J. S., 1949, A histochemical method for localizing Cholinesterase activity. Proc. Soc. Exp. Biol. Med. 70: 617–622.PubMedGoogle Scholar
  102. Kremzner, L. T., and Wilson, I. B., 1963, A chromatographic procedure for the purification of acetylcholinesterase. J. Biol. Chem. 238: 1714–1717.PubMedGoogle Scholar
  103. La Du, B. N., 1971, Plasma esterase activity and the metabolism of drugs with ester groups. Ann. N.Y. Acad. Sci. 179: 684–694.PubMedGoogle Scholar
  104. La Du, B. N., and Lockridge, O., 1986, Molecular biology of human serum Cholinesterase. Fed. Proc. 45: 2965–2969.PubMedGoogle Scholar
  105. Lappin, R. I., and Rubin, L. L., 1985, Molecular forms of acetylcholinesterase in Xenopus muscle. Dev. Biol. 110: 269–274.PubMedGoogle Scholar
  106. Lawler, H. C., 1964, The preparation of a soluble acetylcholinesterase from brain. Biochim. Biophys. Acta 81: 280–288.Google Scholar
  107. Lazar, M., and Vigny, M., 1980, Modulation of the distribution of acetylcholinesterase molecular forms in a murine neuroblastoma sympathetic ganglion cell hybrid cell line. J. Neurochem. 35: 1067–1079.PubMedGoogle Scholar
  108. Lenoir-Rousseaux, J.-J. 1985, Les arthropodes possèdent une même forme moléculaire majoritaire d’acétylcholinestérase. C.R. Soc. Biol. 179: 741–747.Google Scholar
  109. Leuzinger, W., and Baker, A. L., 1967, Acetylcholinesterase. I. Large-scale purification, homogeneity, and amino acid analysis. Proc. Natl. Acad. Sci. U.S.A. 57: 446–451.PubMedGoogle Scholar
  110. Lindenbaum, M. H., and Livett, B. G., 1983, Acetylcholinesterase molecular forms in C57BL/6J dystrophic mice. Muscle Nerve 6: 638–645.PubMedGoogle Scholar
  111. Lockridge, O., 1984, Amino acid composition and sequence of human serum Cholinesterase: A progress report. In Cholinesterases, Fundamental and Applied Aspects (M. Brzin, E. A., Barnard, and D. Sket, eds.), pp. 5–20, Walter de Gruyter, Berlin.Google Scholar
  112. Lockridge, O., and La Du, B. N. J., 1978, Comparison of atypical and usual human serum cholinesterase: Purification, number of active sites, substrate affinity, and turnover number. J. Biol. Chem. 253: 361–366.PubMedGoogle Scholar
  113. Lockridge, O., and La Du, B. N., 1982, Loss of the interchain disulfide peptide and dissociation of the tetramer following limited proteolysis of the native human serum Cholinesterase. J. Biol. Chem. 257: 12012–12018.PubMedGoogle Scholar
  114. Lockridge, O., Eckerson, H. W., and La Du, B. N., 1979, Interchain disulfide bonds and subunit organisation in human serum Cholinesterase. J. Biol. Chem. 254: 8324–8330.PubMedGoogle Scholar
  115. Lockridge, O., Bartels, C. F., Vaughan, T. A., Wang, C. K., Norton, S. E., and Johnson, L. L., 1987, Complete amino acid sequence of human serum Cholinesterase. J. Biol. Chem. 262: 549–557.PubMedGoogle Scholar
  116. Lyles, J. M., Silman, I., and Barnard, E. A., 1979, Developmental changes in levels and forms of cholinesterases in muscles of normal and dystrophic chickens. J. Neurochem. 33: 727–738.PubMedGoogle Scholar
  117. Lyles, J. M., Barnard, E. A., and Silman, I., 1980, Changes in the levels and forms of cholinesterases in the blood plasma of normal and dystrophic chickens. J. Neurochem. 34: 978–987.PubMedGoogle Scholar
  118. Lyles, J. M., Silman, I., Di Giamberardino, L., Couraud, J.-Y., and Barnard, E. A., 1982, Comparison of the molecular forms of the cholinesterases in tissues of normal and dystrophic chickens. J. Neurochem. 38: 1007–1021.PubMedGoogle Scholar
  119. MacPhee-Quigley, K., Taylor, P., and Taylor, S., 1985, Primary structures of the catalytic subunits from two molecular forms of acetylcholinesterase. A comparison of NH2-terminal and active center sequences. J. Biol. Chem. 260: 12185–12189.PubMedGoogle Scholar
  120. Marsh, D., Grassi, J., Vigny, M., and Massoulié, J., 1984, An immunological study of rat acetylcholinesterase: Comparison with acetylcholinesterases from other vertebrates. J. Neurochem. 43: 204–213.PubMedGoogle Scholar
  121. Massoulié, J., and Bon, S., 1976, Affinity chromatography of acetylcholinesterase; the importance of hydrophobic interactions. Eur. J. Biochem. 68: 531–539.PubMedGoogle Scholar
  122. Massoulié, J., and Bon, S., 1982, The molecular forms of Cholinesterase and acetylcholinesterase in vertebrates. Annu. Rev. Neurosci. 5: 57–106.PubMedGoogle Scholar
  123. Massoulié, J., and Rieger, F., 1969, L’acétylcholinestérase des organes électriques de poissons (Torpille et Gymnote). Eur. J. Biochem. 11: 441–455.PubMedGoogle Scholar
  124. Massoulié, J., Bon, S., and Vigny, M., 1980, The polymorphism of Cholinesterase in vertebrates. Neurochem. Int. 2: 161–184.Google Scholar
  125. Mays, C., and Rosenberry, T. L., 1981, Characterization of pepsin-resistant collagen-like tail subunit fragments of 18S and 14S acetylcholinesterase from Electrophorus electricus. Biochemistry 20: 2810–2817.Google Scholar
  126. Méflah, K., Bernard, S., and Massoulié, J., 1984, Interactions with lectins indicate differences in the carbohydrate composition of the membrane-bound enzymes acetylcholinesterase and 5′-nucleotidase in different cell types. Biochimie 66: 59–69.PubMedGoogle Scholar
  127. Meier-Ruge, W., 1968, Das megacolon: seine Diagnose und Pathophysiologie, Virchows Arch. (Pathol. Anat.) 344: 67–85.Google Scholar
  128. Metz, J., Stevens, K., van Rensburg, N. J., and Hart, D., 1961, Failure of in-vivo inhibition of acetylcholinesterase to affect erythrocyte life-span: The significance of the enzyme defect in paroxysmal nocturnal haemoglobinuria. Br. J. Haematol. 7: 458–463.PubMedGoogle Scholar
  129. Milunsky, A., and Sapirstein, V. S., 1982, Prenatal diagnosis of open neural defects using the amniotic fluid acetylcholinesterase assay. Obstet. Gynecol. 59: 1–6.PubMedGoogle Scholar
  130. Mintz, K. P., and Brimijoin, S., 1985a, Two-step immunoaffinity purification of acetylcholinesterase from rabbit brain. J. Neurochem. 44: 225–232.PubMedGoogle Scholar
  131. Mintz, K. P., and Brimijoin, S., 1985b, Monoclonal antibodies to rabbit brain acetylcholinesterase: Selective enzyme inhibition, differential affinity for enzyme forms, and cross-reactivity with other mammalian cholinesterases. J. Neurochem. 45: 284–292.PubMedGoogle Scholar
  132. Mintz, K. P., Weinshilboum, R. M., and Brimijoin, W. S., 1984, Evolution of butyrylcholinesterase in higher primates: An immunochemical study. Comp. Biochem. Physiol. 79C: 35–37.Google Scholar
  133. Muensch, H., Goedde, H. W., and Yoshida, A., 1976, Human serum Cholinesterase subunits and number of active sites of the major component. Eur. J. Biochem. 70: 217–223.PubMedGoogle Scholar
  134. Nicholson-Weller, A., March, J. P., Rosenfield, S. I., and Austen, K. F., 1983, Affected erythrocytes of patients with paroxysmal nocturnal hemoglobinuria are deficient in the complement regulatory protein, decay accelerating factor. Proc. Natl. Acad. Sci. U.S.A. 80: 5066–5070.PubMedGoogle Scholar
  135. Nicholson-Weller, A., Spicer, D. B., and Austen, K. F., 1985, Deficiency of the complement regulatory protein decay accelerating factor on membranes of erythrocytes, monocytes, and platelets in paroxysmal nocturnal hemoglobinuria. N. Engl. J. Med. 312: 1091–1097.PubMedGoogle Scholar
  136. Nicolet, M., Pincon-Raymond, M., and Rieger, F., 1986, Globular and asymmetric acetylcholinesterase in frog muscle basal lamina sheaths. J. Cell Biol. 102: 762–768.PubMedGoogle Scholar
  137. Niday, E., Wang, C. S., and Alaupovic, P., 1977, Studies on the characterization of human erythrocyte acetylcholinesterase and its interaction with antibodies. Biochim. Biophys. Acta 459: 180–193.Google Scholar
  138. Nørgaard-Pedersen, B., Hangaard, J., and Bjerrum, O. J., 1983, Quantitative enzyme antigen immunoassay of acetylcholinesterase in amniotic fluid. Clin. Chem. 29: 1061–1064.PubMedGoogle Scholar
  139. Okamoto, E., and Ueda, T., 1967, Embryogenesis of intramural ganglia of the gut and its relation to Hirschsprung’s disease. J. Pediatr. Surg. 2: 437–443.Google Scholar
  140. Ott, P., Jenny, B., and Brodbeck, U., 1975, Multiple molecular forms of purified human erythrocyte acetylcholinesterase. Eur. J. Biochem. 57: 469–480.PubMedGoogle Scholar
  141. Paggi, P., Scarsella, G., and Toschi, G., 1977, Multiple forms of acetylcholinesterase in the ciliary ganglia and iris of the pigeon. Neuroscience 2: 1085–1093.Google Scholar
  142. Parvari, R., Pecht, I., and Soreq, H., 1983, A microfluorometric assay for cholinesterases suitable for multiple kinetic determinations of picomoles of released thiocholine. Anal. Biochem. 133: 450–456.PubMedGoogle Scholar
  143. Patrick, W. J. A., Besley, G. T. N., and Smith, I. I., 1980, Histochemical diagnosis of Hirschsprung’s disease and a comparison of the histochemical and biochemical activity of acetylcholinesterase in rectal mucosal biopsies. J. Clin. Pathol. 33: 336–343.PubMedGoogle Scholar
  144. Perry, E. K., Perry, R. H., Blessed, G., and Tomlinson, B. E., 1977, Necropsy evidence of central cholinergic deficits in senile dementia. Lancet i: 189.Google Scholar
  145. Perry, E. K., Tomlinson, B. E., Blessed, G., Bergmann, K., Gibson, P. H., and Perry, R. H., 1978, Correlation of cholinergic abnormalities with senile plaques and mental test scores in senile dementia. Br. Med. J. 2: 1457–1459.PubMedGoogle Scholar
  146. Perry, R. H., Candy, J. M., Perry, E. K., Irving, D., Blessed, G., Fairbairn, A. F., and Tomlinson, B. E., 1982, Extensive loss of choline acetyltransferase activity is not reflected by neuronal loss in the nucleus of Meynert in Alzheimer’s disease. Neurosci. Lett. 33: 311–315.PubMedGoogle Scholar
  147. Pilowsky, P. M., Hodgson, A. J., and Chubb, I. W., 1982, Acetylcholinesterase in neural tube defects: A model using chick embryo amniotic fluid. Neuroscience 7: 1203–1214.PubMedGoogle Scholar
  148. Poiana, G., Scarsella, G., Biagioni, S., Senni, M. I., and Cossu, G., 1985, Membrane acetylcholinesterase in murine muscular dystrophy in vivo and in cultured myotubes. Int. J. Dev. Neurosci. 3: 331–340.Google Scholar
  149. Raba, R., Aaviksaar, A., Raba, M., and Siigur, J., 1979, Cobra venom acetylcholinesterase. Purification and molecular properties. Eur. J. Biochem. 96: 151–158.PubMedGoogle Scholar
  150. Rakonczay, Z., 1986, Mammalian brain acetylcholinesterase. In Neuromethods, Volume 5, Neurotransmitter Enzymes (A. A. Boulton, G. B. Baker, and P. H. Yu, eds.), Chap. 9, pp. 319–360, Humana Press, Clifton, NJ.Google Scholar
  151. Rakonczay, Z., and Brimijoin, S., 1985, Immunochemical differences among molecular forms of acetylcholinesterase in brain and blood. Biochim. Biophys. Acta 832: 127–134.PubMedGoogle Scholar
  152. Rakonczay, Z., and Brimijoin, S., 1986, Monoclonal antibodies to rat brain acetylcholinesterase: Comparative affinity for soluble and membrane-associated enzyme and for enzyme from different vertebrate species. J. Neurochem. 46: 280–287.PubMedGoogle Scholar
  153. Rakonczay, Z., and Brimijoin, S., 1987, Monoclonal antibodies to human brain acetylcholinesterase: Properties and applications. Cell. Mol. Neurobiol. (in press).Google Scholar
  154. Rakonczay, Z., and Németh, P., 1984, Change in the distribution of acetylcholinesterase molecular forms in Hirschsprung’s disease. J. Neurochem. 43: 1194–1196.PubMedGoogle Scholar
  155. Rakonczay, Z., Mallol, J., Schenk, H., Vincendon, G., and Zanetta, J.-P., 1981a, Purification and properties of the membrane-bound acetylcholinesterase from adult rat brain. Biochim. Biophys. Acta 657: 243–256.PubMedGoogle Scholar
  156. Rakonczay, Z., Vincendon, G., and Zanetta, J.-P., 1981b, Heterogeneity of rat brain acetylcholinesterase: A study by gel filtration and gradient centrifugation. J. Neurochem. 37: 662–669.PubMedGoogle Scholar
  157. Rakonczay, Z., Sziits, P., and Szabö, J., 1985, Significance of acetylcholinesterase molecular forms in the prenatal diagnosis of neural tube defects. Acta Physiol. Hung. 66: 280–281.Google Scholar
  158. Ralston, J. S., Rush, R. S., Doctor, B. P., and Wolfe, A. D., 1985, Acetylcholinesterase from fetal bovine serum. Purification and characterization of soluble G4 enzyme. J. Biol. Chem. 260: 4312–4318.PubMedGoogle Scholar
  159. Ramírez, G., Gómez-Barriocanal, J., Barat, A., and Rodríguez-Borrajo, C., 1984, Two classes of collagen-tailed molecular forms of acetylcholinesterase. In Cholinesterases, Fundamental and Applied Aspects (M. Brzin, E. A. Barnard, and D. Sket, eds.), pp. 115–128, Walter de Gruyter, Berlin.Google Scholar
  160. Reisine, T. D., Yamamura, H. I., Bird, E. D., Spokes, E. and Enna, S. J., 1978, Pre- and postsynaptic neurochemical alterations in Alzheimer’s disease. Brain Res. 159: 477–481.PubMedGoogle Scholar
  161. Rieger, F., and Vigny, M., 1976, Solubilization and physicochemical characterization of rat brain acetylcholinesterase: Development and maturation of its molecular forms. J. Neurochem. 27: 121–129.PubMedGoogle Scholar
  162. Rieger, F., Bon, S., Massoulié, J., and Cartaud, J., 1973, Observation par microscopie électronique des formes allongées et globulaires de l’acétylcholinestérase de Gymnote (Electrophorus electricus). Eur. J. Biochem. 34: 539–547.PubMedGoogle Scholar
  163. Rieger, F., Bon, S., Massoulié, Cartaud, J., Picard, B., and Benda, P., 1976, Torpedo marmorata acetylcholinesterase; a comparison with the Electrophorus electricus enzyme: Molecular forms, subunits, electron microscopy, immunological relationship. Eur. J. Biochem. 68: 513–521.PubMedGoogle Scholar
  164. Rieger, F., Chételat, R., Nicolet, M., Kamal, L., and Poullet, M., 1980, Presence of tailed, asymmetric forms of acetylcholinesterase in the central nervous system of vertebrates. FEBS Lett. 121: 169–174.PubMedGoogle Scholar
  165. Rieger, F., Shelanski, M. L., and Sidman, R. L., 1983, The multiple molecular forms of acetylcholinesterase in “motor end-plate disease” in the mouse (medj and med allelic forms): Sensitivity of the 10S form to partial or total loss of muscle activity. Exp. Neurol. 79: 299–315.PubMedGoogle Scholar
  166. Rieger, F., Powell, J. A., and Pincon-Raymond, M., 1984, Extensive nerve overgrowth and paucity of the tailed asymmetric form (16S) of acetylcholinesterase in the developing skeletal neuromuscular system of the dysgenic (mdg/mdg) mouse. Dev. Biol. 101: 181–191.PubMedGoogle Scholar
  167. Roberts, W. L., and Rosenberry, T. L., 1985, Identification of covalently attached fatty acids in the hydrophobic membrane-binding domain of human erythrocyte acetylcholinesterase. Biochem. Biophys. Res. Commun. 133: 621–627.PubMedGoogle Scholar
  168. Roberts, W. L., and Rosenberry, T. L., 1986, Selective radiolabelling and isolation of the hydrophobic membrane-binding domain of human erythrocyte acetylcholinesterase. Biochemistry 25: 3091–3098.PubMedGoogle Scholar
  169. Rodriquez-Borrajo, C., Barat, A., and Ramirez, C., 1982, Solubilization of collagen-tailed molecular forms of acetylcholinesterase from several brain areas in different vertebrate species. Neurochem. Int. 4: 563–568.Google Scholar
  170. Rosenberry, T. L., 1985, Structural distinctions among acetylcholinesterase forms. In The Enzymes of Biological Membranes (A. N. Martonosik, ed.), Vol. 3, pp. 403–429, Plenum Press, New York.Google Scholar
  171. Rossor, M., Fahrenkrug, J., Emson, P., Mountjoy, C., Iversen, L., and Roth, M., 1980, Reduced cortical choline acetyltransferase activity in senile dementia of Alzheimer type is not accompanied by changes in vasoactive intestinal polypeptide. Brain Res. 201: 249–253.PubMedGoogle Scholar
  172. Rotoli, B., Robledo, R., Scarpato, N., and Luzzatto, L., 1984, Two populations of erythroid cell progenitors in paroxysmal nocturnal hemoglobinuria. Blood 64: 847–851.PubMedGoogle Scholar
  173. Rotundo, R. L., 1984, Synthesis, assembly, and processing of AChE in tissue cultured muscle. In Cholinesterases, Fundamental and Applied Aspects (M. Brzin, E. A. Barnard, and D. Sket, eds.), pp. 203–217, Walter de Gruyter, Berlin.Google Scholar
  174. Ruberg, M., Rieger, F., Villageois, A., Bonnet, A. M., and Agid, Y., 1986, Acetylcholinesterase and butyrylcholinesterase in frontal cortex and cerebrospinal fluid of demented and non-demented patients with Parkinson’s disease. Brain Res. 362: 83–91.PubMedGoogle Scholar
  175. Rubinstein, H. M., Dietz, A. A., Hodges, L. K., Lubrano, T., and Czebotar, V. 1970, Silent Cholinesterase gene: Variations in the properties of serum enzyme in apparent homozygotes. J. Clin. Invest. 49: 479–486.PubMedGoogle Scholar
  176. Rubinstein, H. M., Dietz, A. A., Lubrano, T., and Garry, P. J., 1976, Ej 1, a quantitative variant at Cholinesterase locus 1: Immunological evidence. J. Med. Genet. 13: 43–45.PubMedGoogle Scholar
  177. Rubinstein, H. M., Dietz, A. A., and Lubrano, T., 1978, Ek 1, another quantitative variant at Cholinesterase locus 1. J. Med. Genet. 15: 27–29.PubMedGoogle Scholar
  178. Sakai, M., Saisu, H., and Abe, T., 1985, Comparison of asymmetric forms of acetylcholinesterase from the electric organ of Narke japonica and Torpedo californica. Eur. J. Biochem. 153: 497–502.Google Scholar
  179. Sakai M., Koshigoe, N., Saisu, H., and Abe, T., 1986, Monoclonal antibodies specific to asymmetric forms of acetylcholinesterase from the electric organ of electric rays. Biomed. Res. 7: 1–5.Google Scholar
  180. Schumacher, M., Camp, S., Maulet, Y., Newton, M., MacPhee-Quigley, K., Taylor, S. S., Friedmann, T., and Taylor, P., 1986, Primary structure of Torpedo californica acetylcholinesterase deduced from its cDNA sequence. Nature 319: 407–409.PubMedGoogle Scholar
  181. Seller, M. J., and Cole, K. J., 1980, Polyacrylamide gel electrophoresis of amniotic fluid cholinesterases: A good prenatal test for neural tube defects. Br. J. Obstet. Gynaecol. 87: 1103–1108.PubMedGoogle Scholar
  182. Silberstein, L., Inestrosa, N. C., and Hall, Z. W., 1982, Aneural muscle cell cultures make synaptic basal lamina components. Nature 295: 143–145.PubMedGoogle Scholar
  183. Silman, I., Di Giamberardino, L., Lyles, J., Couraud, J.-Y., and Barnard, E. A., 1979, Parallel regulation of acetylcholinesterase and pseudoCholinesterase in normal, denervated and dystrophic chicken skeletal muscle. Nature 280: 160–162.PubMedGoogle Scholar
  184. Silver, A., 1974, The Biology of Cholinesterases, North-Holland, Amsterdam.Google Scholar
  185. Skau, K. A., 1983, The acetylcholinesterase abnormality in dystrophic mice is a reflection of a maturational defect. Brain Res. 276: 192–194.PubMedGoogle Scholar
  186. Skau, K. A., 1985, Acetylcholinesterase molecular forms in serum and erythrocytes of laboratory animals. Comp. Biochem. Physiol. 80C: 207–210.Google Scholar
  187. Skau, K. A., and Brimijoin, S., 1980, Multiple molecular forms of acetylcholinesterase in rat vagus nerve, smooth muscle, and heart. J. Neurochem. 35: 1151–1154.PubMedGoogle Scholar
  188. Skau, K. A., and Brimijoin, S., 1981, Abnormal distribution of skeletal muscle acetylcholinesterase molecular forms in dystrophic mice. Exp. Neurol. 74: 111–121.PubMedGoogle Scholar
  189. Sketelj, J., and Brzin, M., 1985, Asymmetric molecular forms of acetylcholinesterase in mammalian skeletal muscles. J. Neurosci. Res. 14: 95–103.PubMedGoogle Scholar
  190. Sketelj, J., McNamee, M. G., and Wilson, B. W., 1978, Effect of denervation on the molecular forms of acetylcholinesterase in normal and dystrophic chickens. Exp. Neurol. 60: 624–629.PubMedGoogle Scholar
  191. Sketelj, J., Sasel, B., Zupancic, N., and Brzin, M., 1983, Plasma acetylcholinesterase in Duchenne muscular dystrophy. Exp. Neurol. 80: 329–336.PubMedGoogle Scholar
  192. Smith, A. D., 1979, Amniotic fluid acetylcholinesterase and neural tube defects: Plea for standardization. Lancet ii: 307–308.Google Scholar
  193. Smith, A. D., Wald, N. J., Chuckle, H. S., Stirrat, G. M., Bobrow, M., and Lagercrantz, H., 1979, Amniotic fluid acetylcholinesterase as a possible diagnostic test for neural tube defects in early pregnancy. Lancet i:685–688.Google Scholar
  194. Soininen, H., Halonen, T., and Riekkinen, P. J., 1981, Acetylcholinesterase activities in cerebrospinal fluid of patients with senile dementia of Alzheimer type. Acta Neurol. Scand. 64: 217 – 224.PubMedGoogle Scholar
  195. Sørensen, K., Gentinetta, R., and Brodbeck, U., 1982, An amphiphile dependent form of human brain caudate nucleus acetylcholinesterase: Purification and properties. J. Neurochem. 39: 1050–1060.PubMedGoogle Scholar
  196. Sørensen, K., Gennari, K., Brodbeck, U., Landauer, P., and Liefländer, M., 1985, Polymorphism and immunochemical cross-reactivity of acetylcholinesterases from the brains of human, dog, hog, bovine, and horse. Comp. Biochem. Physiol. 80C:263–268.Google Scholar
  197. Sørensen, K., Brodbeck, U., Rasmussen, A. G., and Nørgaard-Pedersen, B., 1986, Normal human serum contains two forms of acetylcholinesterase. Clin. Chim. Acta 158: 1–6.PubMedGoogle Scholar
  198. St. Clair, D. M., Brock, D. J. H., and Barron, L., 1986, A monoclonal antibody assay technique for plasma and red cell acetylcholinesterase activity in Alzheimer’s disease. J. Neurol. Sci. 73: 169–176.PubMedGoogle Scholar
  199. Steiger, S., Cardoso de Almeida, M.-L., Blatter, M. C., Brodbeck, U., and Bordier, C., 1986, The membrane-anchoring systems of vertebrate acetylcholinesterase and variant surface glycoproteins of African trypanosomes share a common antigenic determinant. FEBS Lett. 199: 182–186.Google Scholar
  200. Taylor, P., Jones, J. W., and Jacobs, N. M., 1974, Acetylcholinesterase from Torpedo: Characterization of an enzyme species isolated by lytic procedures. Mol. Pharmacol. 10: 78–92.PubMedGoogle Scholar
  201. Tomlinson, B. E., Blessed, G., and Roth, M., 1970, Observations on the brains of demented old people. J. Neurol. Sci. 11: 205–242.PubMedGoogle Scholar
  202. Toutant, J. P., Massoulié, J., and Bon, S., 1985, Polymorphism of pseudoCholinesterase in Torpedo marmorata tissues. Comparative study of the catalytic and molecular properties of this enzyme with acetylcholinesterase. J. Neurochem. 44: 580–592.PubMedGoogle Scholar
  203. Tsuji, S., Rieger, F., Peltre, G., Massoulié, J., and Benda, P., 1972, Acetylcholinesterase du muscle, de la moelle épiniere et du cerveau de gymnote. J. Neurochem. 19: 989–997.PubMedGoogle Scholar
  204. Tucci, A. F., and Seifter, S., 1969, Preparation and properties of porcine parotid butyrylcholinesterase. J. Biol. Chem. 244: 841–849.PubMedGoogle Scholar
  205. Turner, M. J., Cardoso de Almeida, M. L., Gurnett, A. M., Raper, J., and Ward, J., 1985, Biosynthesis, attachment and release of variant surface glycoproteins of the African trypanosome. Curr. Top. Microbiol. Immunol. 117: 23–55.PubMedGoogle Scholar
  206. Verp, M. S., Milunsky, A., Simpson, J. L., Graham, A., and Sabbagha, R., 1984, Elevated alpha-fetoprotein and acetylcholinesterase associated with hydrocele. Am. J. Med. Genet. 19: 651–652.PubMedGoogle Scholar
  207. Vigny, M., Di Giamberardino, L., Couraud, J.-Y., Rieger, F., and Koenig, J., 1976, Molecular forms of chicken acetylcholinesterase: Effect of denervation. FEBS Lett. 69: 277–280.PubMedGoogle Scholar
  208. Vigny, M., Bon, S., Massoulié, J., and Leterrier, F., 1978, Active site catalytic efficiency of acetylcholinesterase molecular forms in Electrophorus, Torpedo, rat and chicken. Eur. J. Biochem. 85: 317–323.PubMedGoogle Scholar
  209. Vigny, M., Martin, G. R., and Grotendorst, G. R., 1983, Interactions of asymmetric forms of acetylcholinesterase with basement membrane components. J. Biol. Chem. 258: 8794–8798.PubMedGoogle Scholar
  210. Warkany, J., 1971, Congenital Malformations, Notes and Comments, Year Book Medical Publishers, Chicago.Google Scholar
  211. Wenthold, R. J., Mahler, H. R., and Moore, W. J., 1974, Properties of acetylcholinesterase from rat brain. J. Neurochem. 22: 945–949.PubMedGoogle Scholar
  212. Whitehouse, P. J., Price, D. L., Clark, A. W., Coyle, J. T., and Delong, M. R., 1981, Alzheimer’s disease: Evidence for selective loss of cholinergic neurons in the nucleus basalis. Ann. Neurol. 10: 122–126.PubMedGoogle Scholar
  213. Whitehouse, P. J., Price, D. L., Struble, R. G., Coyle, J. T., and Delong, M. R., 1982, Alzheimer’s disease and senile dementia—loss of neurons in the basal forebrain. Science 215: 1237–1239.PubMedGoogle Scholar
  214. Williams, R. M., 1969, Antibodies to acetylcholinesterase. Proc. Natl. Acad. Sci. U.S.A. 62: 1175 – 1180.PubMedGoogle Scholar
  215. Wilson, B. W., Montgomery, M. A., and Asmundson, R. V., 1968, Cholinesterase activity and inherited muscular dystrophy of the chicken. Proc. Soc. Exp. Biol. Med. 129: 199–206.PubMedGoogle Scholar
  216. Younkin, S. G., Rosenstein, C., Collins, P. L., and Rosenberry, T. L., 1982, Cellular localization of the molecular forms of acetylcholinesterase in rat diaphragm. J. Biol. Chem. 257: 13630–13637.PubMedGoogle Scholar
  217. Younkin, S. G., Goodridge, B., Katz, J., Lockett, G., Nafziger, D., Usiak, M. F., and Younkin, L. H., 1986, Molecular forms of acetylcholinesterase in Alzheimer’s disease. Fed. Proc. 45: 2982–2988.PubMedGoogle Scholar
  218. Zanetta, J.-P., Rakonczay, Z., Reeber, A., Kása, P., and Vincendon, G., 1981, Antibodies against the membrane-bound acetylcholinesterase from adult rat brain. FEBS Lett. 129: 293–296.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Zoltán Rakonczay
    • 1
  • Stephen Brimijoin
    • 2
  1. 1.Central Research LaboratoryMedical UniversitySzegedHungary
  2. 2.Department of PharmacologyMayo ClinicRochesterUSA

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