Abstract
The ability to introduce DNA into cells has been a sine-gua-non for the developments in molecular biology. One of the most unusual and unexpected technological breakthroughs in this arena is biolistic transformation. This technique goes under several names, including microparticle injection, gene gun, particle bombardment, etc. The basic idea, as originally conceived of by Sanford and coworkers (1), is disarmingly simple. DNA or other biological material is coated onto heavy metal microprojectiles and the microprojectiles are shot into the target cells, carrying the DNA in with them.
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Johnston, S.A., Tang, DC. (1993). The Use of Microparticle Injection to Introduce Genes into Animal Cells In Vitro and In Vivo . In: Setlow, J.K. (eds) Genetic Engineering. Genetic Engineering: Principles and Methods, vol 15. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1666-2_11
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DOI: https://doi.org/10.1007/978-1-4899-1666-2_11
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