Abstract
Biologically active molecules are often present in minute quantity. Much effort and many samples are required to isolate such molecules and to analyze their structures. When sugar chains are analyzed as molecules with biological activity, a method is needed to isolate and analyze sugar chains with a high sensitivity. Therefore, we developed the pyridylamination method (Hase et al., 1978) for sugar chains and improved this method to apply it to sugar chains from glycoproteins (Hase et al., 1984).
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References
Hase, S. and Ikenaka, T., 1990 Estimation of elution times on reverse-phase high-performance liquid chromatography of pyridylamino derivatives of sugar chains from glycoproteins, Anal. Biochem. 184:135.
Hase, S., Hatanaka, K., Ochiai, K. and Schimizu, H., 1992 Improved method for the component sugar analysis of glycoproteins by pyridylamino sugars purified with immobilized boronic acid, Biosci. Biotech. Biochem. 56, in press.
Hase, S., Ibuki, T. and Ikenaka, T., 1984 Reexamination of the pyridylamination used for fluorescence labeling of oligosaccharides and its application to glycoproteins, J. Biochem. 95:197.
Hase, S., Ikenaka, K., Mikoshiba, K. and Ikenaka, T., 1988 Analysis of tissue glycoprotein sugar chains by two-dimensional high-performance liquid Chromatographic mapping, J. Chromatogr. Biomedical Applications 434:51.
Hase, S., Ikenaka, T. and Matsushima, Y., 1978 Structure analyses of oligosaccharides by tagging of the reducing end sugars with a fluorescent compound, Biochem. Biophys. Res. Commun. 85:257.
Hase, S., Kikuchi, N., Ikenaka, T. and Inoue, K., 1985 Structures of sugar chains of the third component of human complement, J. Biochem. 98:863.
Hase, S., Koyama, S., Daiyasu, H., Takemoto, H., Hara, S., Kobayashi, Y., Kyogoku, Y. and Ikenaka, T., 1986 Structure of a sugar chain of a protease inhibitor isolated from Barbados pride (Caesalpinia pulcherrima Sw.) seeds, J. Biochem. 100:1.
Hase, S., Natsuka, S., Oku, H. and Ikenaka, T., 1987 Identification method for twelve oligomannose-type sugar chains thought to be processing intermediates of glycoproteins, Anal. Biochem. 167:321.
Hase, S., Sugimoto, T., Takemoto, H., Ikenaka, T. and Schmid, K., 1986 The structure of sugar chains of Japanese quail ovomucoid. The occurrence of oligosaccharides not expected from the classical biosynthetic pathway for N-glycans; a method for the assessment of the structure of glycans present in picomolar amounts, J. Biochem. 99:1725.
Kuraya N. and Hase, S., 1992 Release of O-linked sugar chains from glycoproteins with anhydrous hydrazine and pyridylamination of the sugar chains with improved reaction conditions, J. Biochem. 112:122.
Lee, C. Y., Lee, B. I., Tomiya, N. and Takahashi, N., 1990 Parameterization of contribution of sugar units to elution volumes in reverse-phase HPLC of 2-pyridylaminated oligosaccharides, Anal Biochem. 188:259.
Tomiya, N., Awaya, J., Kurono, M., Endo, S., Arata, Y. and Takahashi, N., 1988 Analyses of relinked oligosaccarides using a two-dimensional mapping technique, Anal. Biochem. 171:73.
Yamamoto, S., Hase, S., Fukuda, S., Sano, O. and Ikenaka, T., 1989 Structures of the sugar chains of interferon-γ produced by human myelomonocyte cell line HBL-38, J. Biochem. 105:547.
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© 1993 Springer Science+Business Media New York
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Hase, S. (1993). Pyridylamination for Sensitive Analysis of Sugar Chains of Glycoproteins. In: Imahori, K., Sakiyama, F. (eds) Methods in Protein Sequence Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1603-7_25
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DOI: https://doi.org/10.1007/978-1-4899-1603-7_25
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