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Cyanogen Bromide Cleavage of Proteins on Blots and Subsequent Separation of the Fragments by Polyacrylamide Gel Electrophoresis Directly from those Blots

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Abstract

Since nucleic acid sequencing is now quick and accurate, the usual goal of protein sequencing is no longer to determine an entire primary structure. A partial sequence is sufficient for the design of the oligonucleotide probes, needed to obtain starting material for sequence determinations on the nucleic acid level.

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© 1993 Springer Science+Business Media New York

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Frank, G., Redweik, U., Rümbeli, M., Schoedon, G., Blau, N. (1993). Cyanogen Bromide Cleavage of Proteins on Blots and Subsequent Separation of the Fragments by Polyacrylamide Gel Electrophoresis Directly from those Blots. In: Imahori, K., Sakiyama, F. (eds) Methods in Protein Sequence Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1603-7_10

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  • DOI: https://doi.org/10.1007/978-1-4899-1603-7_10

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1605-1

  • Online ISBN: 978-1-4899-1603-7

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