Abstract
A method was evaluated for the quantitation and identification of fumonisins by on-line liquid chromatography/mass spectrometry (LC/MS) with electrospray ionization. A linear response in the full-scan mode with positive ion detection was obtained for fumonisin B1 (FB1) over the range of 5–5000 ng injected on-column. Purified FB1, FB2, and half-hydrolyzed FB1 showed equimolar responses. Fully hydrolyzed FB1 did not show a similar molar response profile and produced a signal which was approximately 2 times that obtained for an equal quantity of FB1. Most known fumonisins and preparative by-products such as methyl esters were chromatographically resolved and identified by MS by using an acetonitrile gradient and positive ion detection. Negative ion electrospray was used to differentiate fumonisin amides from esters on the basis of differences in response. Response factors for FB1 and the acetyl amide of FB1 in the negative ion mode at pH 4.5 were approximately 1:3, respectively.
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References
Bezuidenhout, S.C.; Gelderblom, W.C.A.; Gorst-Allan, C.P.; Horak, R.M.; Marasas, W.F.O.; Spiteller, G.; Vleggaar, R. Structure elucidation of the fumonisins, mycotoxins from Fusarium moniliforme. J. Chem. Soc. Chem. Commun. 1988, 743-745.
Branham, B.E.; Plattner, R.D. Isolation and characterization of a new fumonisin from liquid cultures of Fusarium moniliforme. J. Nat. Prod. 1993, 56, 1630–1633.
Bruins, A.P.; Covey, T.R.; Henion, J.D. Ion spray interface for combined liquid chromatography/atmospheric pressure ionization mass spectrometry. Anal. Chem. 1987, 59, 2642–2646.
Cawood, M.E.; Gelderblom, W.C.A.; Vleggaar, R.; Behrend, Y.; Thiel, P.G.; Marasas, W.F.O. Isolation of the fumonisin mycotoxins: A quantitative approach. J. Agric. Food Chem. 1991, 39, 1958–1962.
Colvin, B.M.; Harrison, L.R. Fumonisin induced pulmonary edema and hydrothorax in swine. Mycopathologia. 1992, 117, 79–82.
Doerge, D.R., Howard, P.C.; Bajic, S.; Preece, S. Determination of fumonisins using on-line liquid chromatography coupled to electrospray mass spectrometry. Rapid Commun. Mass Spectrom. 1994, 8, 603–606.
Gelderblom, W.C.A.; Cawood, M.E.; Snyman, S.D.; Vleggaar, R.; Marasas, W.F.O. Structure-activity relationships of fumonisins in short-term carcinogensis and cytotoxicity assays. Food Chem. Toxicol. 1993, 31, 407–414.
Holcolm, M.; Thompson, H.C., Jr.; Lipe, G.; Hankins, L.J. HPLC with electrochemical and fluorescence detection of the OPA/2-methyl-2-propanethiol derivative of fumonisin B1. J. Liq. Chromatogr. 1994, 17, 4121–4129.
Hopmans, E.C.; Murphy, P.A. Detection of fumonisins B1, B2, and B3 and hydrolyzed fumonisin FB1 in corn-containing foods. J. Agric. Food Chem. 1993, 41, 1655–1658.
Kellerman, T.S.; Marasas, W.F.O.; Thiel, P.G.; Gelderblom, W.C.A.; Cawood, M.E.; Coetzer, J.A.W. Leukoencephalomalacia in two horses induced by oral dosing of FB1. Onderstepoort J. Vet. Res. 1990, 57, 269–275.
Korfmacher, W.A.; Chiarelli, M.P.; Lay, J.O., Jr.; Bloom, J.; Holcolb, M.; McManus, K.T. Characterization of the mycotoxin fumonisin B1: Comparison of thermospray, fast-atom bombardment and electrospray mass spectrometry. Rapid Commun. Mass Spectrom. 1991, 5, 463–468.
Plattner, R.D.; Norred, W.P.; Bacon, C.W.; Voss, K.A., Peterson, R.; Shackelford, D.D.; Weisleder, D. Amethod of detection of fumonisins in corn samples associated with field cases of equine leukoencephalomalacia. Mycologia. 1990, 82, 698–702.
Plattner, R.D.; Weisleder, D.; Shackelford, D.D.; Peterson, R.; Powell, R.G. A new fumonisin from solid cultures of Fusarium moniliforme. Mycopathologia. 1992, 117, 23–28.
Ross, P.F.; Nelson, P.E.; Richard, J.L.; Osweiler, G.D.; Rice, L.G.; Plattner, R.D.; Wilson, T.M. Production of fumonisins by Fusarium moniliforme and Fusarium proliferatum isolates associated with equine leukoencephalomalacia and pulmonary edema in swine. Appl. Environ. Microbiol. 1990, 56, 3225–3226.
Scott, P.M.; Lawrence, G.A. Stability and problems in recovery of fumonisins added to corn-based foods. J. AOAC Int. 1994, 77, 541–545.
Stack, M.E.; Eppley, R.M. Liquid Chromatographie determination of fumonisins B, and B2 in corn and corn products. J. AOAC Int. 1992, 75, 834–836.
Thakur, R.A.; Smith, J.S. Analysis of fumonisin B1 by negative-ion thermospray mass spectrometry. Rapid Commun. Mass Spectrom. 1994, 8, 82–88.
Young, J.C.; Lafontaine, P. Detection and characterization of fumonisin mycotoxins as their methyl esters by liquid chromatography/particle beam mass spectrometry. Rapid Commun. Mass Spectrom. 1993, 7, 352–359.
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Musser, S.M. (1996). Quantitation and Identification of Fumonisins by Liquid Chromatography/Mass Spectrometry. In: Jackson, L.S., DeVries, J.W., Bullerman, L.B. (eds) Fumonisins in Food. Advances in Experimental medicine and Biology, vol 392. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1379-1_6
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DOI: https://doi.org/10.1007/978-1-4899-1379-1_6
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