Abstract
Several methods are presently used to identify and quantify fumonisins in foods and feeds. HPLC procedures on derivatized fumonisins with fluorescence detection are most commonly used. The validity and significance of reported fumonisin levels depend on several factors such as the specificity, detection limit, accuracy and reproducibility of the analytical method as well as on the sampling procedure used, and the integrity and purity of the analytical standards. The importance of these factors is discussed and the results of two international collaborative studies are presented on the determination of fumonisins in corn by a reversed-phase HPLC method on o-phthaldialdehyde (OPA) derivatized fumonisins using fluorescence detection.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Azcona-Olivera, J.I.; Abouzied, M.M.; Plattner, R.D.; Norred, W.P.; Pestka, J.J. Generation of antibodies reactive to fumonisins B1, B2, and B3 by using cholera toxin as the carrier-adjuvant. Appl. Environ. Microbiol. 1992a, 58, 169–173.
Azcona-Olivera, J.I.; Abouzied, M.M.; Plattner, R.D.; Pestka, J.J. Production of monoclonal antibodies to the mycotoxins fumonisins B1, B2, and B3. J. Agric. Fd Chem. 1992b, 40, 531–534.
Bennet, G.A.; Richard, J.L. Liquid Chromatographic method for analyses of the naphthalene dicarboxaldehyde derivative of fumonisins. J. Assoc. Off Anal. Chem. Int. 1994, 77, 501–505.
Bezuidenhout, S.C.; Gelderblom, W.C.A.; Gorst-Allman, C.P.; Horak, R.M.; Marasas, W.F.O.; Spiteller, G.; Vleggaar, R. Structure elucidation of the fumonisins, mycotoxins from Fusarium moniliforme. J. Chem. Soc. Chem. Commun. 1988, 743-745.
Doerge, D.R.; Bajic, S.; Preece, S.W.; Howard, P.C. Determination of fumonisins using on-line liquid chromatography coupled to electrospray mass spectrometry. Rapid Commun. Mass Spectrom. 1994, 8, 603–606.
Gelderblom, W.C.A.; Jaskiewicz, K.; Marasas, W.F.O.; Thiel, P.G.; Horak, R.M.; Vleggaar, R.; Kriek, N.P.J. Fumonisins-novel mycotoxins with cancer-promoting activity produced by Fusarium moniliforme. Appl. Environ. Microbiol. 1988, 54, 1806–1811.
Gelderblom, W.C.A.; Kriek, N.P.J.; Marasas, W.F.O.; Thiel, P.G. Toxicity and carcinogenicity of the Fusarium moniliforme metabolite, fumonisin B1, in rats. Carcinogenesis 1991, 12, 1247–1251.
Gelderblom, W.C.A.; Cawood, M.E.; Snyman, D.; Vleggaar, R.; Marasas, W.F.O. Structure-activity relationships of fumonisins in short-term carcinogenesis and cytotoxicity assays. Fd. Chem. Toxicol. 1993, 31, 407–414.
Harrison, L.R.; Colvin, B.M.; Greene, J.T.; Newman, L.E.; Cole, J.R. Pulmonary edema and hydrothorax in swine produced by fumonisin B1, a toxic metabolite of Fusarium moniliforme. J. Vet. Diagn. Invest. 1990, 2, 217–221.
Holcomb, M; Sutherland, J.B.; Chiarelli, M.P.; Korfmacher, W.A.; Thompson, H.C. Jr.; Lay, J.O. Jr.; Hankins, L.J.; Cerniglia, C.E. HPLC and FAB mass spectrometry analysis of fumonisins B1 and B2 produced by Fusarium moniliforme on food substrates. J. Agric. Fd Chem. 1993a, 41, 357–360.
Holcomb, M.; Thompson, H.C. Jr.; Hankins, L.J. Analysis of fumonisin B1 in rodent feed by gradient elution HPLC using precolumn derivatization with FMOC and fluorescence detection. J. Agric. Fd Chem. 1993b, 41, 764–767.
Horwitz, W.; Albert, R. Performance characteristics of methods of analysis used for regulatory purposes. Part II. Pesticide Formulations. J. Assoc. Off. Anal Chem. 1991, 74, 718–744.
Horwitz, W.; Albert, R.; Nesheim, S. Reliability of mycotoxin assays-An update. J. Assoc. Off Anal. Chem. 1993, 76, 461–491.
Kellerman, T.S.; Marasas, W.F.O.; Thiel, P.G.; Gelderblom, W.C.A.; Cawood, M.E.; Coetzer, J.A.W. Leukoencephalomalacia in two horses induced by oral dosing of fumonisin B1. Onderstepoort J. Vet. Res. 1990, 57, 269–275.
Korfmacher, W.A.; Chiarelli, M.P.; Lay, J.O. Jr.; Bloom, J.; Holcomb, M.; McManus, K.T. Characterization of the mycotoxin fumonisin B1: comparison of thermospray, fast-atom bombardment and electrospray mass spectrometry. Rapid Commun. Mass Spectrom. 1991, 5, 463–468.
Maragos, C.M.; Richard, J.L. Quantification and stability of fumonisins B1 and B2 in milk. J. Assoc. Off. Anal. Chem. Int. 1994, 77, 1162–1167.
Miller, J.D.; Savard, M.E.; Sibilia, A.; Rapior, S.; Hocking, A.D.; Pitt, J.I. Production of fumonisins and fusarins by Fusarium moniliforme from Southeast Asia. Mycologia 1993, 85, 385–391.
Norred, W.P.; Voss, K.A.; Bacon, C.W.; Riley, R.T. Effectiveness of ammonia treatment in detoxification of fumonisin-contaminated corn. Fd Chem. Toxicol. 1991, 29, 815–819.
Pestka, J.J.; Azcona-Olivera, J.I.; Plattner, R.D.; Minervini, F.; Doko, M.B.; Visconti, A. Comparative assessment of fumonisin in grain-based foods by ELISA, GC-MS, and HPLC. J. Fd Prot. 1994, 57, 169–172.
Plattner, R.D.; Norred, W.P.; Bacon, C.W.; Voss, K.A.; Peterson, R.; Shackelford, D.D.; Weisleder, D. A method of detection of fumonisins in corn samples associated with field cases of equine leukoencephalomalacia. Mycologia 1990, 82, 698–702.
Plattner, R.D.; Weisleder, D.; Shackelford, D.D.; Peterson, R.; Powell, R.G. A new fumonisin from solid cultures of Fusarium moniliforme. Mycopathologia 1992, 117, 23–28.
Plattner, R.D.; Branham, B.E. Labelled fumonisins: Production and use of fumonisin B1 containing stable isotopes. J. Assoc. Off. Anal. Chem. Int. 1994, 77, 525–532.
Riley, R.T.; An, N-H.; Showker, J.L.; Yoo, H-S.; Norred, W.P.; Chamberlain, W.J.; Wang, E.; Merrill, A.H.; Motelin, G.; Beasley, V.R.; Haschek, WM. Alteration of tissue and serum sphinganine to sphingosine ratio: An early biomarker of exposure to fumonisin-containing feeds in pigs. Toxicol. Appl. Pharmacol. 1993, 118, 105–112.
Ross, P.F.; Rice, L.G.; Plattner, R.D.; Osweiler, G.D.; Wilson T.M.; Owens, D.L.; Nelson, H.A.; Richard, J.L. Concentrations of fumonisin B1 in feeds associated with animal health problems. Mycopathologia 1991, 114, 129–135.
Ross, P.F.; Nelson, P.E.; Owens, D.L.; Rice, L.G.; Nelson, H.A.; Wilson, T.M. Fumonisin B2 in cultured Fusarium proliferatum, M-6104, causes equine leukoencephalomalacia. J. Vet. Diagn. Invest. 1994, 6, 263–265.
Rottinghaus, G.E.; Coatney, C.E.; Minor, H.C. A rapid, sensitive thin layer chromatography procedure for the detection of fumonisin B1 and B2. J. Vet. Diagn. Invest. 1992, 4, 326–329.
Scott, P.M.; Lawrence, G.A. Liquid Chromatographic determination of fumonisins with 4-fluoro-7-nitrobenzofurazan. J. Assoc. Off. Anal. Chem. Int. 1992, 75, 829–834.
Scott, P.M.; Lawrence, G.A. Stability and problems in recovery of fumonisins added to corn-based foods. J. Assoc. Off. Anal. Chem. Int. 1994, 77, 541–545.
Shelby, R.A.; Rottinghaus, G.E.; Minor, H.C. Comparison of thin-layer chromatography and competitive immunoassay methods for detecting fumonisin on maize. J. Agric. Fd Chem. 1994, 42, 2064–2067.
Shephard, G.S.; Sydenham, E.W.; Thiel, P.G.; Gelderblom, W.C.A. Quantitative determination of fumonisins B1 and B2 by highperformance liquid chromatography with fluorescence detection. J. Liq. Chromatogr. 1990, 13, 2077–2087.
Shephard, G.S.; Thiel, P.G.; Sydenham, E.W.; Alberts, J.F.; Cawood, M.E. Distribution and excretion of a single dose of the mycotoxin fumonisin B1 in a non-human primate. Toxicon 1994, 32, 735–741.
Stockenström, S.; Sydenham, E.W.; Thiel, P.G. Determination of fumonisins in corn: evaluation of two purification procedures. Mycotoxin Res. 1994, 10, 9–14.
Sydenham, E.W.; Gelderblom, W.C.A.; Thiel, P.G.; Marasas, W.F.O. Evidence for the natural occurrence of fumonisin B1, a mycotoxin produced by Fusarium moniliforme in corn. J. Agric. Fd Chem. 1990, 38, 285–290.
Sydenham, E.W.; Shephard, G.S.; Thiel, P.G. Liquid Chromatographic determination of fumonisin B1, B2 and B3 in foods and feeds. J. Assoc. Off. Anal. Chem. 1992, 75, 313–318.
Sydenham, E.W.; Van der Westhuizen, L.; Stockenström, S.; Shephard, G.S.; Thiel, P.G. Fumonisin-contaminated maize: physical treatment for the partial decontamination of bulk shipments. Food Addit. Contam. 1994, 11, 25–32.
Thiel, P.G.; Shephard, G.S.; Sydenham, E.W.; Marasas, W.F.O.; Nelson, P.E.; Wilson, T.M. Levels of fumonisin B1 and B2 in feeds associated with confirmed cases of equine leukoencepha lomalacia. J. Agric. Fd. Chem. 1991, 39, 109–111.
Thiel. P.G.; Marasas, W.F.O.; Sydenham, E.W.; Shephard, G.S.; Gelderblom, W.C.A. The implications of naturally occurring levels of fumonisins in corn for human and animal health. Mycopathologia 1992, 777, 3–9.
Thiel, P.G.; Sydenham, E.W.; Shephard, G.S.; van Schalkwyk, D.J. Study of the reproducibility characteristics of a liquid Chromatographic method for the determination of fumonisins B1 and B2 in corn: IUPAC collaborative study. J. Assoc. Off. Anal. Chem. Int. 1993, 76, 361–366.
Usleber, E.; Straka, M.; Terplan, G. Enzyme immunoassay for fumonisin B1 applied to corn-based food. J. Agric. Fd Chem. 1994, 42, 1392–1396.
Voss, K.A.; Chamberlain, W.J.; Bacon, C.W.; Norred, W.P. A preliminary investigation on renal and hepatic toxicity in rats fed purified fumonisin B1. Natural Toxins 1993, 1, 222–228.
Wang, E.; Norred, W.P; Bacon, C.W.; Riley, R.T.; Merrill, A.H. Inhibition of sphingolipid biosynthesis by fumonisins: implications for diseases associated with Fusarium moniliforme. J. Biol. Chem. 1991, 266, 14486–14490.
Ware, G.M.; Umrigar, P.P.; Carman, A.S. Jr.; Kuan, S.S. Evaluation of fumonitest immunoaffinity columns. Anal. Lett. 1994, 27, 693–715.
Wilson, T.M.; Ross, P.F.; Owens, D.L.; Rice, L.G.; Jenkins, S.J.; Nelson, H.A. Experimental production of ELEM. A study to determine the minimum toxic dose in ponies. Mycopathologia 1992, 117, 115–120.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer Science+Business Media New York
About this chapter
Cite this chapter
Thiel, P.G., Sydenham, E.W., Shephard, G.S. (1996). The Reliability and Significance of Analytical Data on the Natural Occurrence of Fumonisins in Food. In: Jackson, L.S., DeVries, J.W., Bullerman, L.B. (eds) Fumonisins in Food. Advances in Experimental medicine and Biology, vol 392. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1379-1_13
Download citation
DOI: https://doi.org/10.1007/978-1-4899-1379-1_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-1381-4
Online ISBN: 978-1-4899-1379-1
eBook Packages: Springer Book Archive