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Fumonisins Veratox®

A New Rapid Quantitative ELISA for Determination of Fumonisin in Food and Feed

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Fumonisins in Food

Part of the book series: Advances in Experimental medicine and Biology ((AEMB,volume 392))

Abstract

Polyclonal antibodies against fumonisin B1 were produced by immunizing sheep with fumonisin B1-keyhole limpet hemocyanin as an immunogen. A quantitative competitive enzyme-linked immunosorbent assay was developed whereby free fumonisins or sample extract containing fumonisins and enzyme-labelled fumonisin competed for binding to the solid phase-bound antibodies. The color intensity of wells, formed by substrate reaction with the enzyme, was inversely related to FB1 concentration. Detection limits for the assay were 0.1 ng/mL fumonisin B1 and concentrations of fumonisins B1, B2, and B3 required for 50% binding inhibition were 5.5, 23 and 18 ng/mL, respectively. For food and feed analyses, samples were extracted with 70% methanol and dilutions of the extracts were used directly for ELISA. ELISA results were compared to HPLC analyses by a reference laboratory and the correlation (r value) between ELISA and HPLC was 0.967. The assay may be used to quantitate fumonisins in food and feed samples within 30 minutes.

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Authors and Affiliations

  1. Neogen Corporation, Lansing, MI, 48912, USA

    Mohamed M. Abouzied, Scott D. Askegard, Charles B. Bird & Brinton M. Miller

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  1. Mohamed M. Abouzied
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  2. Scott D. Askegard
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  3. Charles B. Bird
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  4. Brinton M. Miller
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Editor information

Editors and Affiliations

  1. Food and Drug Administration, National Center for Food Safety and Technology, Summit-Argo, Illinois, USA

    Lauren S. Jackson

  2. General Mills, Inc., Minneapolis, Minnesota, USA

    Jonathan W. DeVries

  3. University of Nebraska-Lincoln, Lincoln, Nebraska, USA

    Lloyd B. Bullerman

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© 1996 Springer Science+Business Media New York

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Abouzied, M.M., Askegard, S.D., Bird, C.B., Miller, B.M. (1996). Fumonisins Veratox®. In: Jackson, L.S., DeVries, J.W., Bullerman, L.B. (eds) Fumonisins in Food. Advances in Experimental medicine and Biology, vol 392. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1379-1_12

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  • DOI: https://doi.org/10.1007/978-1-4899-1379-1_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1381-4

  • Online ISBN: 978-1-4899-1379-1

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