Purification and Properties of UDP-Glucose : Diosgenin/Tigogenin Glucosyltransferase from Solanum melongena Leaves

  • Cezary Paczkowski
  • Zdzislaw A. Wojciechowski
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 404)


Spirostanol 3-O-oligosides (steroid saponins) are widely distributed in the plant kingdom, particularly in species belonging to the Liliaceae, Scrophulariaceae, Dios­coreaceae, Zygophyllaceae, and Solanaceae families1,2. The importance of these glycosides for chemical protection of plants against microbial pathogens and/or herbivores has been suggested3,4. The frequently observed fungitoxic, antiviral, cytostatic, and hypocholestero­lemic activities6 of steroid saponins showed that these compounds are of more than academic interest. There are, however, only sparse data on the formation of steroid saponins in plants, especially at the enzyme level. The major part of information concerning the biosynthesis of these compounds is derived from experiments in which more or less distant labeled precursors (e.g. acetate, mevalonate, or cholesterol) were fed to the intact plants, plant organs, or cell cultures7,8. These experiments have elucidated in outline the biosynthetic pathways leading to the formation of spirostanol aglycones but until recently almost nothing was known about the synthesis of sugar chains of steroid saponins.


Ammonium Sulfate Cytosol Fraction Sugar Chain Steroid Saponin Acetone Powder 
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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Cezary Paczkowski
    • 1
  • Zdzislaw A. Wojciechowski
    • 1
  1. 1.Department of BiochemistryWarsaw UniversityWarszawaPoland

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