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Biological Activity of Saponins from Two Dracaena Species

  • C. O. Okunji
  • M. M. Iwu
  • J. E. Jackson
  • J. D. Tally
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 404)

Abstract

Many species of the west African “soap tree” Dracaena are used in traditional medicine for the treatment of a variety of diseases. In continuation of our search for anti-infective agents from plants implicated in traditional medicine, we evaluated the biological activities of saponins from extracts of Dracaena mannii and Dracaena arborea by using a battery of test systems such as radiorespirometry, Cytosensor®, bioautography, and agar dilution methods and molluscicidal tests.

Bioassay-directed fractionation of the methanol extracts of seed pulp using a combination of chromatographic techniques, gel filtration, droplet countercurrent chromatography (DCCC), and low-pressure liquid chromatography (Lobar), led to the isolation and characterization of spiroconazole A, a pennogenin triglycoside [3β-O-{(α-L-rhamnopyranosyl(1→2), α-L-rhamnopyranosyl(1→3)-β-D-glucopyranosyl}-17α-hydroxylspirost-5-ene] (Fig. 1). As the active constituent, spiroconazole A exhibited pronounced antileishmanial, antimalarial, and molluscicidal activities. This paper also reports on the fungistatic, fungicidal and bacteriostatic activity of spiroconazole A against 17 species of fungi and 4 of bacteria.

Keywords

Visceral Leishmaniasis Cutaneous Leishmaniasis Fruit Pulp Agar Dilution Method Minimum Fungicidal Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • C. O. Okunji
    • 1
    • 2
  • M. M. Iwu
  • J. E. Jackson
  • J. D. Tally
  1. 1.Division of Experimental TherapeuticsWalter Reed Army Institute of ResearchUSA
  2. 2.NRC Senior Research AssociateUniversity of NigeriaNsukkaNigeria

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