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(Post-Column) Reaction-Detection: An Alternative to Improve Sensitivity and Selectivity in Column Liquid Chromatographic Analysis

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Sample Preparation for Biomedical and Environmental Analysis

Part of the book series: Chromatographic Society Symposium Series ((CSSS))

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Summary

In sample preparation, in principle, two routes can be followed to improve selectivity and sensitivity. The most frequently applied is the rigorous removal of all interfering substances. This means, however, that in many cases time-consuming and laborious procedures must be used. The other route is the use of a specific detection technique for the analyte in the crude sample. Although this approach is not always possible chemical manipulations can be useful as a means of achieving this aim. Among these techniques, post-column reaction-detection systems are frequently used in combination with column liquid chromatographic (CLC) separations.

A wide variety of post-column reaction-detection techniques can be used. Examples are presented with respect to the use of on-line (electrochemical) generation of bromine for the detection of thiols, the hydrolytic reaction of barbiturates, the photolysis of dansylated phenols, the copper-containing reactor for the determination of thiram, and the immobilised enzyme reactor in combination with chemiluminescence detection.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands

    H. Lingeman & U. A. Th. Brinkman

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  1. H. Lingeman
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  2. U. A. Th. Brinkman
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Editors and Affiliations

  1. The Robens Institute of Industrial and Environmental Health and Safety, University of Surrey, Guildford, Surrey, UK

    D. Stevenson

  2. Zeneca Pharmaceuticals, Macclesfield, Cheshire, UK

    I. D. Wilson

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© 1994 Springer Science+Business Media New York

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Lingeman, H., Brinkman, U.A.T. (1994). (Post-Column) Reaction-Detection: An Alternative to Improve Sensitivity and Selectivity in Column Liquid Chromatographic Analysis. In: Stevenson, D., Wilson, I.D. (eds) Sample Preparation for Biomedical and Environmental Analysis. Chromatographic Society Symposium Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1328-9_3

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  • DOI: https://doi.org/10.1007/978-1-4899-1328-9_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1330-2

  • Online ISBN: 978-1-4899-1328-9

  • eBook Packages: Springer Book Archive

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