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Morphogenesis in Plants pp 221–236Cite as

Gene Expression in Mesophyll Protoplasts

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Part of the book series: NATO ASI Series ((NSSA,volume 253))

Abstract

Higher plants are multicellular organisms, composed of tissues and organs specialised in particular functions. As a consequence, due to their position in the plant, cells present various morphologies, have different metabolisms and each cell type expresses a particular set of genes. Nevertheless, under particular circumstances, this differentiation can be lost. For example, after wounding cells reexpress their ability to grow and form a healing callus. If the damage is extensive, some cells localised near the wound reform stem or root meristems (decapitated plants, cuttings). In some plant species shoot and root meristem neoformation is not limited to wound repair, but is the basis of the natural plant propagation. Thus cell dedifferentiation is an important process in plant development. It is also the basis of in vitro tissue culture, since most cell lines are obtained from primary expiants composed of differentiated cells. Nevertheless, the process of cell dedifferentiation in the entire plant or in large primary expiants cultivated in vitro is difficult to analyse, due to the heterogeneity of the cell population. Protoplasts isolated from leaf mesophyll constitute a primary expiant which is composed of a pippetable population of cells, all originating from the same tissue. In culture, most protoplasts follow the same development and divide.

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Authors and Affiliations

  1. Laboratoire de Biologie Moléculaire et Physiologie Végétale, Unité associée au CNRS 565, Université avenue de Villeneuve, 66860, Perpignan, France

    Yves Meyer, Yvette Chartier, Jean Grosset, Isabelle Marty, Christophe Brugidou, Paulo Marinho & Renata Rivera

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  1. Yves Meyer
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  2. Yvette Chartier
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  3. Jean Grosset
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  4. Isabelle Marty
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  5. Christophe Brugidou
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  1. University of Crete, Heraklion, Greece

    Kalliopi A. Roubelakis-Angelakis

  2. CNRS, Gif-sur-Yvette, France

    Kiem Tran Van Thanh

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Meyer, Y. et al. (1993). Gene Expression in Mesophyll Protoplasts. In: Roubelakis-Angelakis, K.A., Van Thanh, K.T. (eds) Morphogenesis in Plants. NATO ASI Series, vol 253. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1265-7_12

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