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New Concepts of a Blood—Brain Barrier pp 119–140Cite as

Long-Chain Fatty Acid Transport at the Blood-Brain Barrier and Incorporation into Brain Phospholipids: A New In Vivo Method for Examining Neuroplasticity, and Brain Second Messenger Systems Involving Phospholipase A2 Activation

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Abstract

Brain functional activity and structural integrity are related to rates of formation and turnover of phospholipids (Porcellati et al., 1983; Fisher and Agranoff, 1987; Axelrod et al., 1988). Until recently, these rates were estimated by injecting radiolabeled precursors intracerebrally in animals — radiolabeled glucose, acetate, ethanolamine, glycerol, fatty acids (FAs) or ethanolamine — and by killing the animals at different times thereafter to follow biochemical compartment radioactivities (Sun and Su, 1979; Porcellati et al., 1987). Unfortunately, intracerebral injections damage brain tissue, are not applicable to autoradiographic analysis or in vivo positron emission tomography (PET), and give half-lives of acyl groups within phospholipids which ignore effects of recycling.

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Author information

Authors and Affiliations

  1. Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Bethesda, Maryland, 20892, USA

    S. I. Rapoport

  2. Miami Valley Laboratories, The Proctor and Gamble Company, Cincinnati, OH, 45239-8707, USA

    P. J. Robinson

Authors
  1. S. I. Rapoport
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  2. P. J. Robinson
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Editors and Affiliations

  1. Institute of Ophthalmology, University College London, London, England

    John Greenwood

  2. King’s College, University of London, London, England

    David J. Begley

  3. United Medical and Dental Schools, St. Thomas’ Hospital, London, England

    Malcolm B. Segal

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Rapoport, S.I., Robinson, P.J. (1995). Long-Chain Fatty Acid Transport at the Blood-Brain Barrier and Incorporation into Brain Phospholipids: A New In Vivo Method for Examining Neuroplasticity, and Brain Second Messenger Systems Involving Phospholipase A2 Activation. In: Greenwood, J., Begley, D.J., Segal, M.B. (eds) New Concepts of a Blood—Brain Barrier. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1054-7_13

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  • DOI: https://doi.org/10.1007/978-1-4899-1054-7_13

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1056-1

  • Online ISBN: 978-1-4899-1054-7

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