Abstract
The amino acid sequence of a protein of interest is usually one of the first pieces required in today’s molecular biology, be it for gene cloning or synthesis of immunoreactive peptides. To date, amino(N)-terminal sequencing using the Edman degradation procedure has almost exclusively provided such data. Methodologies for sequencing proteins from their carboxy(C)-termini have remained relatively primitive requiring much protein in return for little sequence information. Carboxypeptidase digestion is still the most widely used method despite its intrinsic limitations of substrate specificity and endoprotease contamination. Several chemical degradation methods have been reported (Stark, 1968; Yamashita,1971; Bailey et al., 1994), and a few automated C-terminal sequencers are almost available to the public.
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References
Amons, R., 1987, Vapor-phase modification of sulfhydryl group in proteins, FEBS Lett. 212: 68–72.
Bailey, J.M., Tu, O., Issai, G., Ha, A., and Shively, J.E., 1994, C-terminal sequence analysis of polypeptides containg C-terminal protein, Protein Society Poster No.151-S
Biemann, K., 1987 (Pub. 1988 ), Contribution of mass spectrometry to peptide and protein structure, Biomed. Environ. Mass Spectrom. 16: 99–111.
Boyd, R.K., and Beynon, J.H., 1977, Scanning of sector mass spectrometers to observe the fragmentation of metastable ions, Org. Mass Spectrom. 12: 163–165.
Edman, P.and Begg, G., 1967, A protein sequenator, Eur. J. Biochem. 1: 80–91.
Gaskell, S.J., Pike, A.W., and Millington, D.S., 1979, Selected metastable peak monitoring. A new, specific technique in quantitative gas chromatography-mass spectrometry, Biomed. Mass Spectrom. 6: 78–81.
Nabuchi, Y., Yano, H., Kamo, M., Takamoto, K., Satake, K. & Tsugita, A., 1994, C-terminal sequencing of peptdes and proteins by successive degradation with heptafluorobutyric anhydride vapor, Chem. Lett. 1994: 757–760.
Naylor, S., Findeis, A.F., Gibson, B.W., and Williams, D.H., 1996, An approach toward the complete FAB analysis of enzymic digests of peptide and proteins, J. Am. Chem. Soc. 108: 6359–6363.
Stark, G.R., 1968, Sequential degradation of peptides from their carboxy termini with ammonium thiocyanate and acetic anhydride, Biochem. 7: 1796–1807.
Tsugita, A., Takamoto, K., Kamo, M., and Iwadate, H., 1992a, C-terminal sequencing of protein. A novel acid hydrolysis and analysis by mass spectrometry, Eur. J. Biochem. 206: 691–696.
Tsugita, A., Takamoto, K., and Satake, K., 1992b, Reaction of pentafluoropropionic anhydride vapor on polypeptides as revealed by mass spectrometry. A carboxypeptidase mimetic degradation, Chem. Lett. 1992: 235–238.
Tsugita, A., Takamoto, K., Iwadate, H., Kamo, M., and Satake, K., 1992c, A novel protein C-terminal sequencing method using mass spectrometry, in Biological Mass Spectrometry ( Matsuo, T., eds) pp. 242–243, San-ei pub., Kyoto.
Tsugita, A., Takamoto, K., Iwadate, H., Kamo, M., Yano, H., Miyatake, N., and Satake, K., 1993, Development of novel C-terminal sequencing methods, in Methods in Protein Sequence Analysis. ( Imahori, K., & Sakiyama, F., eds) pp. 55–62, Plemnum Pub., New York.
Yamashita, S., 1971, Sequential degradation of polypeptides from the carboxyl ends. I. Specific cleavage of the carboxyl-end peptide bonds, Biochem. Biophys. Acta. 229: 301–309.
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Takamoto, K., Kamo, M., Satake, K., Tsugita, A. (1995). A C-Terminal Sequencing Method Using Perfluoroacyl Anhydrides Vapor. In: Atassi, M.Z., Appella, E. (eds) Methods in Protein Structure Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1031-8_9
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DOI: https://doi.org/10.1007/978-1-4899-1031-8_9
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