A C-Terminal Sequencing Method Using Perfluoroacyl Anhydrides Vapor
The amino acid sequence of a protein of interest is usually one of the first pieces required in today’s molecular biology, be it for gene cloning or synthesis of immunoreactive peptides. To date, amino(N)-terminal sequencing using the Edman degradation procedure has almost exclusively provided such data. Methodologies for sequencing proteins from their carboxy(C)-termini have remained relatively primitive requiring much protein in return for little sequence information. Carboxypeptidase digestion is still the most widely used method despite its intrinsic limitations of substrate specificity and endoprotease contamination. Several chemical degradation methods have been reported (Stark, 1968; Yamashita,1971; Bailey et al., 1994), and a few automated C-terminal sequencers are almost available to the public.
KeywordsFast Atom Bombardment Mass Spectrometry Fast Atom Bombardment Mass Spectrum Nitrobenzyl Alcohol Successive Degradation CNBr Cleavage
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