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High Sensitivity Analysis of PTH Amino Acids

  • Karen C. Waldron
  • Michael Carpenter
  • Ian Ireland
  • Darren Lewis
  • Xing Fang Li
  • Norman J. Dovichi

Abstract

Capillary electrophoresis and laser-based photothermal detection are used to analyze minute amounts of PTH amino acids. This technology is demonstrated for analysis of manual Edman degradation reactions. This technology is also used to analyze the products generated by a highly miniaturized automated protein sequencer.

Keywords

Capillary Electrophoresis Reaction Chamber Edman Degradation High Sensitivity Analysis Insulin Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    P. Edman, Acta Chem. Scand. 4, 283–293 (1950).CrossRefGoogle Scholar
  2. 2.
    R. M. Hewick, M. W. Hunkapiller, L. E. Hood, and W. J. Dreyer (1981). J. Biol. Chem., 256 (15), 7990–7997.PubMedGoogle Scholar
  3. 3.
    M. Haniu, and J. E. Shively (1988). Anal. Biochem., 173, 296–306.PubMedCrossRefGoogle Scholar
  4. 4.
    N. F. Totty, M. D. Waterfield, and J. J. Hsuan, (1992). Protein Science, 1, 1215–1224.PubMedCrossRefGoogle Scholar
  5. 5.
    K. C. Waldron, and N. J. Dovichi, (1992). Anal. Chem., 64 (13), 1396–1399.CrossRefGoogle Scholar
  6. 6.
    K. Otsuka, S. Terabe, and T. Ando, (1985). J. Chromatogr., 332, 219–226.CrossRefGoogle Scholar
  7. 7.
    M. Chen, K.C. Waldron, Y. Zhao and N.J. Dovichi Electrophoresis in press.Google Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Karen C. Waldron
    • 1
  • Michael Carpenter
    • 1
  • Ian Ireland
    • 1
  • Darren Lewis
    • 1
  • Xing Fang Li
    • 1
  • Norman J. Dovichi
    • 1
  1. 1.Department of ChemistryUniversity of AlbertaEdmontonCanada

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