Abstract
Over the past three decades, considerable effort has been focused on elucidating the mechanisms by which polypeptide chains fold into well-defined three-dimensional structures (Kim & Baldwin, 1990; Creighton, 1992a; Mattthews, 1993). Major goals of these studies include the identification and characterization of partially folded intermediates and the analysis of transition states that represent the energetic barriers in the folding mechanism. Recently, there has been great progress in the structural analysis of folding intermediates by high resolution NMR spectroscopy of intermediate analogs and native proteins that have been isotopically-labeled during refolding. Structural analysis alone, however, is not sufficient to determine why particular intermediates form or what types of interactions stabilize their conformations. By their very nature, transition states are even more difficult to characterize directly. Questions about folding energetics and the roles of individual interactions in determining the folding mechanism can often be addressed by studying the folding of protein variants that differ by relatively small perturbations of the covalent structure. Recently-developed genetic techniques have made it possible to alter virtually any amino acid residue in a protein, and mutational methods have now been used to study the folding mechanisms of several proteins (Fersht et al., 1992; Goldenberg 1992a; Jennings et al., 1992). We describe here some of our recent work using amino acid replacements to study the folding of a particularly well-characterized protein, bovine pancreatic trypsin inhibitor (BPTI).
Keywords
- Direct Reduction
- Amino Acid Replacement
- Folding Pathway
- Folding Intermediate
- Bovine Pancreatic Trypsin Inhibitor
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Goldenberg, D.P., Mendoza, J.A., Zhang, JX. (1995). Mutational Analysis of the BPTI Folding Pathway. In: Atassi, M.Z., Appella, E. (eds) Methods in Protein Structure Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1031-8_44
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DOI: https://doi.org/10.1007/978-1-4899-1031-8_44
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