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The Human DNA-Activated Protein Kinase, DNA-PK: Substrate Specificity

  • Carl W. Anderson
  • Margery A. Connelly
  • Susan P. Lees-Miller
  • Lauri G. Lintott
  • Hong Zhang
  • John D. Sipley
  • Kazuyasu Sakaguchi
  • E. Appella

Abstract

Agents that cause damage to DNA (DNA damage-inducing (DDI) agents) arrest cell cycle progression in all eukaryotes from yeast to humans at positions in late G1 and G2 that have become known as “checkpoints” (Hartwell and Weinert, 1989; Murray, 1992; Sheldrick and Carr, 1993; Weinert and Lydall, 1993), presumably to allow time for DNA repair. Otherwise the DNA damage would become irreversibly fixed as a consequence of DNA replication in S phase, or through cell division at mitosis (M phase). The mammalian G2 checkpoint mechanism is not yet well characterized (O’Connor and Kohn, 1992), but the key observation that tumor cells with mutant p53 were unable to arrest in G1 (Kastan et al., 1991) quickly led to an outline of the mammalian G1 checkpoint mechanism (Hunter, 1993; Appella and Anderson, 1994; Appella et al., this volume). The p53 tumor suppressor gene is a transcription factor that normally is relatively inactive because it is rapidly degraded (Levine, 1993). However, in response to exposure to ultraviolet light, ionizing radiation, and other DDI agents, the p53 protein is transiently stabilized, accumulates in the cell nucleus, and induces the expression of several genes including WAF1 and GADD45 (El-Deiry et al., 1993; El-Deiry et al., 1994). The 21 kDa product of WAF1 is a potent inhibitor of the cyclin-dependent protein kinases that are needed for the transition from G1 to S phase and for continued DNA replication in S (Dulie et al., 1994; Harper et al., 1993). Although the G1 checkpoint mechanism probably is much more complex, the induction of WAF1 provides a simple explanation of how cell cycle progression can be arrested. In addition to WAF1, about 50 other genes are known to be induced in mammalian cells after exposure to DDI agents (Fornace, 1992; Herrlich and Rahmsdorf, 1994). Recent studies indicate that some genes are induced as a consequence of the effects of DDI agents on other cellular molecules and not necessarily as a consequence of damage to DNA (Anderson, 1994; Herrlich, and Rahmsdorf, 1994; Sachsenmaier et al., 1994). Such exposures activate cytoplasmic signaling mechanisms that operate through protein kinase cascades initiated at or near the plasma membrane; in turn, these kinase cascades activate several transcription factors including AP1 and NF-κB. Nevertheless, there is strong evidence that the p53-dependent induction of WAF1 is a direct consequence of the production of DNA strand breaks (Nelson and Kastan, 1994) and that DNA strand breaks are the signals for activation of the G1 checkpoint(s) in yeast (Siede et al., 1994).

Keywords

Peptide Substrate Serum Response Factor Peptide Phosphorylation Arrest Cell Cycle Progression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Carl W. Anderson
    • 1
  • Margery A. Connelly
    • 1
  • Susan P. Lees-Miller
    • 2
  • Lauri G. Lintott
    • 2
  • Hong Zhang
    • 1
  • John D. Sipley
    • 1
  • Kazuyasu Sakaguchi
    • 3
  • E. Appella
    • 3
  1. 1.Biology DepartmentBrookhaven National LaboratoryUptonUSA
  2. 2.Department of Biological SciencesUniversity of CalgaryN.W., CalgaryCanada
  3. 3.Laboratory of Cell BiologyNational Institutes of HealthBethesdaUSA

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