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Function and Structure of Human Leucocyte Collagenase

  • H. Tschesche
  • V. Knäuper
  • T. Kleine
  • P. Reinemer
  • S. Schnierer
  • F. Grams
  • W. Bode

Abstract

Human leucocyte collagenase is one member of the growing protein family of matrix metalloproteinases (MMPs) [Knäuper et al., 1990]. It is a calcium-containing Zn-endoproteinase (MMP-8) that cleaves preferentially interstitial native triple-helical type I but also type II and type III collagen into one-quarter and three quarter fragments of the native chain length. If thus differs from the fibroblast interstitial collagenase that preferentially cleaves type III. About one-third of its mass of 65 kDa (for active enzyme) is carbohydrates in contrast to the homologous interstitial collagenase from fibroblasts which carries only a small carbohydrate portion [Tschesche et al., 1992]. The enzyme is stored in the specific granules of granulocytes and is released as a proenzyme, also designated latent enzyme, upon stimulation of the cells by various chemotactic agents, such as formylpeptides, LTB4, C5a, Fla and Zymosan amongst others, [Tschesche et al., 1989 and 1991]. Extracellular activation is then achieved by various different proteinases, such as trypsin, kallikrein, chymotrypsin, cathepsin G [Tschesche et al., 1992] or stromelysin [Knäuper et al., 1993]. However, the physiological process of activation is not yet fully understood, since activation was also observed by isolated leucocyte membranes [Tschesche unpublished].

Keywords

Catalytic Domain Type Versus Collagen Specific Granule Active Site Cleft Interstitial Collagenase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • H. Tschesche
    • 1
  • V. Knäuper
    • 1
  • T. Kleine
    • 1
  • P. Reinemer
    • 2
  • S. Schnierer
    • 1
  • F. Grams
    • 2
  • W. Bode
    • 2
  1. 1.Lehrstuhl für Biochemie Fakultät ChemieUniversität BielefeldBielefeldGermany
  2. 2.Max-Planck-Institut für BiochemieMartinsriedGermany

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