Abstract
The virion of the filamentous bacteriophage fd (fl and M13 are very similar strains) is a flexible rod about 1 μm long and 6 nm in diameter, comprising a tubular sheath of approx. 2700 copies of the major coat protein subunit surrounding a DNA core. The DNA is a single-stranded circular molecule of 6408 nucleotides embodying 10 genes; these genes are tightly packed and, in some instances, overlapping, apart from a short region (the intergenic space) which encodes no protein component but which contains a double-stranded helical hairpin loop responsible for initiating assembly of the virion. There are a few copies (about 5) of each of two minor coat proteins at the two ends of the virion: gVIIp and gIXp at the end where assembly is initiated, and glllp and gVIp at the end where the process is terminated [for general reviews, see Model & Russe1,1988; Russel, 1991)].
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Perham, R.N., Marvin, D.A., Symmons, M.F., Welsh, L.C., Terry, T.D. (1995). DNA-Protein Interactions and Protein-Protein Interactions in Filamentous Bacteriophage Assembly. In: Atassi, M.Z., Appella, E. (eds) Methods in Protein Structure Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1031-8_30
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DOI: https://doi.org/10.1007/978-1-4899-1031-8_30
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