Investigation of Protein-Protein Interactions in Mitochondrial Steroid Hydroxylase Systems Using Site-Directed Mutagenesis
Adrenodoxin belongs to the family of /2Fe-2S/ type ferredoxins being widely distributed in bacteria, plants and animals. Although adrenodoxin is a small (~14 kDa) and soluble protein, its three-dimensional structure has not been elucidated as yet. It functions as an electron carrier from the FAD-containing NADPH-dependent ferredoxin reductase to the cytochromes P450scc (CYP11A1), which catalyzes the side-chain cleavage of cholesterol, the initial step in adrenal steroidogenesis, and P45011β (CYP11B1), being involved in the formation of cortisol and aldosterone (Fig. 1).
KeywordsRedox Partner Ferredoxin Reductase Cholesterol Side Chain Cleavage Redox Potential Measurement Adrenodoxin Reductase
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