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Purification Scheme for Isolation and Identification of Peptides Cross-Linked to the rRNA in Ribosomes

  • Henning Urlaub
  • Volker Kruft
  • Brigitte Wittmann-Liebold

Abstract

Ribosomes are complex organelles which contain 50 to 80 different ribosomal proteins (r-proteins) and several RNA molecules (rRNAs). Bacteria sediment at 70S with 30S and 50S subunits whereas eukaryotes have a sedimentation coefficient of 80S with 40S and 60S subunits. Accordingly, the ribosomes from different organisms vary considerably although their main function, namely to perform the translation of the genetic message into proteins is generally maintained. Without detailed knowledge of the molecular structure of the components and their topography within the organelle the translational processes in which more than 200 molecules are involved cannot be understood. Therefore, information on the nearest neighborhoods of the RNAs and the r-proteins and their individual domains is the basis for understanding the translational machinery on a molecular level.

Keywords

Ribosomal Protein Size Exclusion Chromatography Amino Acid Level Ribosomal Subunit Purification Scheme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Henning Urlaub
    • 1
  • Volker Kruft
    • 1
  • Brigitte Wittmann-Liebold
    • 1
  1. 1.ProteinchemieMax-Delbrück-Centrum für Molekulare MedizinBerlin-BuchGermany

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