Abstract
X-ray photoelectron spectroscopy (XPS) is a surface sensitive analytical technique which measures the binding energy of electrons in atoms and molecules. The binding energy can be related to the molecular bonding or oxidation state of an element in the outermost layer of a material, that is 100 A. Thus, XPS is able to identify chemical species present on the surface of a molecule. In this paper XPS is briefly described. Spectra demonstrating its potential use for probing the surface properties of amino acids, polypeptides, proteins, carbohydrates and glycoproteins are discussed.
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References
Carlson, T. A., 1975 in Photoelectron and Auger Spectroscopy, Plenum Press, NY.
Robinson, J. W., 1991 in Practical Handbook of Spectroscopy, CRC Press, Boca Raton, FL.
Worley, C. M., Vannet, M. D., Ball, G. L. and Moddeman, W. E., 1987 Surface chemistry of a microcoated energetic material, pentaerythritoltetranitrate ( PETN ). Surface and Interface Analysis, 10: 273.
Chiu, D., Tappel, A.L. and Millard, M.M., 1977 Improved procedure for x-ray photoelectron spectroscopy of selenium-glutathione peroxidase and application to the rat liver enzyme. Arch. Biochem. Biophys. 184: 209.
Meisenheimer, R. G., Fisher, J. W. and Rehfeld, S. J., 1976 Thallium in human erythrocyte membranes: an x-ray photoelectron spectroscopy study. Biochem. Biophys. Res. Commun. 68, 994.
Pickart, L., Millard, M. M., Beiderman, B. and Thaler, M. M., 1978 Surface analysis and depth profiles of calcium in hepatoma cells during pyruvate-induced synthesis. Biochim. Biophys. Acta, 544: 138.
Millard, M. M., Scherrer, R. and Thomas, R. S. 1976 Surface analysis and depth profile composition of bacterial cells by x-ray photoelectron spectroscopy and oxygen plasma etching. Biochem. Biophys. Res. Commun. 72: 1209.
Peeling, J., Clark, D. T., Evans, M. and Boulter, D. 1976 Evaluation of the ESCA technique as a screening method for the estimation of protein content and quality in seed meals. J. Sci. Fd. Agric. 27: 331.
Siegbahn, K., Mordling, C., Fahlman, A, Mordbert, R., Hedman, J., Johnsson, G., Bergmark, T., Karlsson, S. E., Lindgren, I. and Linberg, B., 1967 ESCA-Atomic, Molecular, and Solid State Structure Studied by Means of Electron spectroscopy, Nova Acta Regiae Soc. Sci. Upsaliensis Ser. IV Vol. 20.
Bumben, K. D. and Dev, S. B. 1988 Investigation of poly(L-amino acids) by x-ray photoelectron spectroscopy. Anal. Chem. 60: 1393.
Clark, D. T., Peeling, J. and Colling, L., 1976 An experimental and theoretical investigation of the core level spectra of a series of amino acids, dipeptides and polypeptides. Biochim. Biophys. Acta 453: 533.
Scofield, J. H., 1973 in Theoretical cross-sections from 1–1500 KeV, Lawrence Livermore Laboratory Report UCRL-51326.
Gendler, S. J., Spicer, A. P., Lalani, E. -N, Duhig, T., Peat, N., Burchell, J., Pemberton, L., Boshell, M. and Taylor-Papadimitriou, J., 1991 Structure and biology of a carcinoma-associated mucin, MUC 1. Amer. Rev. Resp. Dis. 144: S42.
Hanisch, F. -G., Uhlenbruck, G., Peter-Katalinic, J., Egge, H., Dabrowski, J. and Dabrowski, U., 1989 Structures of neutral 0-linked polylactosaminoglycans on human skim milk mucins. J. Biol. Chem. 264: 872.
Gerken, T. A., Gupta, R and Jentoft, N., 1992 A novel approach for chemically deglycosylating 0-linked glycoproteins. The deglycosylation of submaxillary and respiratory mucins. Biochemistry 31: 639.
Gupta, R and Jentoft, N., 1989 Subunit structure of porcine submaxillary mucin. Biochemistry 28: 6114.
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© 1995 Springer Science+Business Media New York
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Dombrowski, K.E., Wright, S.E., Birkbeck, J.C., Moddeman, W.E. (1995). X-Ray Photoelectron Spectroscopy of Amino Acids, Polypeptides and Simple Carbohydrates. In: Atassi, M.Z., Appella, E. (eds) Methods in Protein Structure Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1031-8_22
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DOI: https://doi.org/10.1007/978-1-4899-1031-8_22
Publisher Name: Springer, Boston, MA
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