Abstract
C9 is the last acting protein in the complement cascade. Upon interaction with the membrane bound precursor complex, composed of C5b, C6, C7, and C8, C9 undergoes a conformational change from a monomeric globular plasma protein into an oligomeric integral membrane protein, thus generating an amphiphilic channel across the lipid bilayer of the target cell.
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References
Bidlingmeyer, B. A., Cohen, S. A., and Tarvin, T. L., 1984, Rapid analysis of amino acids using pre-column derivatization, J. Chromatogr. 336: 93.
Biesecker, G., and Mueller-Eberhard, H. J., 1980, The ninth component of human complement: purification and physiochemical characterization, J. Immunol. 124: 1291.
Chang, J.-Y., and Knecht, R., 1991, Direct analysis of the disulfide content of proteins: methods for monitoring the stability and refolding process of cystine-containing proteins, Anal. Biochem. 197: 52.
Dankert, J. R., Shiver, J. W., and Esser, A. F., 1985, Ninth component of complement: selfaggregation and interaction with lipids, Biochemistry 24: 2754.
DiScipio, R. G., Gehring, M. R., Podack, E. R., Kan, C. C., Hugli, T. E., and Fey, G. H., 1984, Nucleotide sequence of rDNA and derived amino acid sequence of human complement C9, Proc. Natl. Acad. Sci. U.S.A. 81: 7298.
DiScipio, R. G., and Hugli, T. E., 1989, The molecular architecture of human complement component C6, J. Biol. Chem. 264: 16197.
Haefliger, J.-A., Tschopp, J., Vial, N., and Jenne, D. E., 1989, Complete primary structure and functional characterization of the sixth component of the human complement system, J. Biol. Chem. 264: 18041.
Hess, D., Schaller, J., and Rickli, E. E., 1991, Identification of the disulfide bonds of human complement C 1 s, Biochemistry 30: 2827.
Marazziti, D., Eggertsen, G., Fey, G. H., and Stanley, K. K., 1988, Relationships between the gene and protein structure in human complement component C9, Biochemistry 27: 6529.
Savage, C. R., Hash, J H., and Cohen, S., 1973, Epidermal growth factor, J. Biol. Chem. 248: 7669.
Sim, R. B., Day, A. J., Moffat, B. E., and Fontaine, M., 1993, Complement factor I and cofactors in control of complement system convertase enzymes, Methods Enzymol. 223: 13–35.
Stanley, K. K., Kocher, H. P., Luzio, J. P., Jackson, P., and Tschopp, J., 1985, The sequence and topology of human complement C9, EMBO J. 4: 375.
Sueyoshi, T., Miyata, T., Iwanaga, S., Toko’oka, T., and Imai, K., 1985, Application of a fluorogenic reagent, ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate for detection of cystine-containing peptides, J. Biochem. (Tokyo) 97: 1811.
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© 1995 Springer Science+Business Media New York
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Lengweiler, S., Schaller, J., Rickli, E.E. (1995). Identification of the Disulfide Bonds of Human Complement Component C9. In: Atassi, M.Z., Appella, E. (eds) Methods in Protein Structure Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1031-8_19
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DOI: https://doi.org/10.1007/978-1-4899-1031-8_19
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