Tracing Cell Signalling Pathways Using a Combination of 2D Gel Electrophoresis and Mass Spectrometry

  • Manfredo Quadroni
  • Chantal Corti
  • Werner Staudenmann
  • Ernesto Carafoli
  • Peter James

Abstract

Many complex biological processes such as cell growth and differentiation are under the control of hormones, some of which act through membrane bound receptors altering the phosphorylation state of the proteins involved in the transformation, regulating their activity. The arrival of only a few hormone molecules to a specific plasma membrane receptor produces, after several steps of amplification, a phosphorylation of a few billion target molecules. In order to study the phosphorylation states of the target proteins and to follow the steps leading to their phosphorylation, an extremely high resolution separation method coupled with an equally sensitive detection method is needed.

Keywords

Casein Kinase Plasma Membrane ATPase Petroselinum Crispum Clone Lambda Deconvoluted Mass Spectrum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Manfredo Quadroni
    • 1
  • Chantal Corti
    • 1
  • Werner Staudenmann
    • 1
  • Ernesto Carafoli
    • 1
  • Peter James
    • 1
  1. 1.Protein Chemistry and Biochemistry III LaboratoriesSwiss Federal Institute of TechnologyZurichSwitzerland

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