Tracing Cell Signalling Pathways Using a Combination of 2D Gel Electrophoresis and Mass Spectrometry
Many complex biological processes such as cell growth and differentiation are under the control of hormones, some of which act through membrane bound receptors altering the phosphorylation state of the proteins involved in the transformation, regulating their activity. The arrival of only a few hormone molecules to a specific plasma membrane receptor produces, after several steps of amplification, a phosphorylation of a few billion target molecules. In order to study the phosphorylation states of the target proteins and to follow the steps leading to their phosphorylation, an extremely high resolution separation method coupled with an equally sensitive detection method is needed.
KeywordsCasein Kinase Plasma Membrane ATPase Petroselinum Crispum Clone Lambda Deconvoluted Mass Spectrum
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