Automated C-Terminal Sequencing of Polypeptides Containing C-Terminal Proline
There has been much interest in the development of a chemical method for the sequential C-terminal sequence analysis of proteins and peptides. Such a method would be analogous and complimentary to the Edman degradation commonly used for N-terminal sequence analysis (Edman, 1950). It would also be invaluable for the sequence analysis of proteins with naturally occurring N-terminal blocking groups, for the detection of post-translational processing at the carboxy-terminus of expressed gene products, and for assistance in the design of oligonucleotide probes for gene cloning. Although a number of methods have been described, the method known as the “thiocyanate method” (Schlack and Kumpf, 1926), has been the most widely studied and appears to offer the most promise due to its similarity to current methods of N-terminal sequence analysis.
KeywordsTrifluoroacetic Acid Acetic Anhydride Quaternary Amine Ammonium Thiocyanate Common Amino Acid
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