C-Terminal Protein Sequence Analysis Using the Hewlett-Packard G1009A C-Terminal Protein Sequencing System
An automated carboxy-terminal (C-terminal) protein sequencing technology developed by Hewlett-Packard enables the direct confirmation of the C-terminal sequence of native and expressed proteins, the detection and characterization of protein processing at the C-terminus, the identification of post-translational proteolytic cleavages, and partial sequence information on amino-terminally blocked protein samples. The approach offers sequence analysis through each of the 20 common amino acid residues including proline, which has historically been highly problematic. Additionally, the scope of typically analyzable protein samples spans a usefully broad molecular weight range and degree of structural complexity.
KeywordsHuman Serum Albumin Reaction Membrane Reaction Support Fluoroacetic Acid Aqueous Trifluoroacetic Acid
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