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C-Terminal Protein Sequence Analysis Using the Hewlett-Packard G1009A C-Terminal Protein Sequencing System

  • Chad G. Miller
  • Jerome M. Bailey
  • David H. Hawke
  • Sherrell Early
  • Jacqueline Tso

Abstract

An automated carboxy-terminal (C-terminal) protein sequencing technology developed by Hewlett-Packard enables the direct confirmation of the C-terminal sequence of native and expressed proteins, the detection and characterization of protein processing at the C-terminus, the identification of post-translational proteolytic cleavages, and partial sequence information on amino-terminally blocked protein samples. The approach offers sequence analysis through each of the 20 common amino acid residues including proline, which has historically been highly problematic. Additionally, the scope of typically analyzable protein samples spans a usefully broad molecular weight range and degree of structural complexity.

Keywords

Human Serum Albumin Reaction Membrane Reaction Support Fluoroacetic Acid Aqueous Trifluoroacetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bailey, J. M., Nikfarjam, F., Shenoy, N. S., and Shively, J. E., 1992 Automated carboxy-terminal sequence analysis of peptides and proteins using diphenyl phophoroisothiocyanatidate, Protein Science 1: 1622.PubMedCrossRefGoogle Scholar
  2. HP G1009A C-Terminal Protein Sequencing System technical note (1994) TN 94–5.Google Scholar
  3. Bailey, J. M. and Shively, J. E., 1990 Carboxy-terminal sequencing: formation and hydrolysis of C-terminal peptidylthiohydantoins, Biochemistry 29: 3145.PubMedCrossRefGoogle Scholar
  4. Bailey, J. M., Tu, O., Issai, G., and Shively, J. E., 1995 C-terminal sequence analysis of polypeptides containing C-terminal proline, in: Techniques in Protein Chemisty VI, Crabb, J.W. ed., Academic Press, San Diego, in press.Google Scholar
  5. Miller, C.G., 1995 Adsorptive biphasic column technology for protein sequence analysis and protein chemical modification, in: Methods: A Companion to Methods in Enzymology, Shively, J.E. ed., Academic Press, San Diego, in press.Google Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Chad G. Miller
    • 1
  • Jerome M. Bailey
    • 1
  • David H. Hawke
    • 1
  • Sherrell Early
    • 1
  • Jacqueline Tso
    • 1
  1. 1.Protein Chemistry SystemsHewlett-Packard CompanyPalo AltoUSA

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