Fatigue pp 175-184 | Cite as

Bioenergetics and Muscle Cell Types

  • M. J. Kushmerick
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 384)


Potential complexities in biochemical and bioenergetic interpretation due to fiber type heterogeneity are not significant for human muscle. Paradigms for understanding muscle bioenergetics then can be understood from a set of basic premises of biochemical energy balance 1) ATP provides the energy for all forms of muscle work; 2) chemical energy is stored in cells as phosphocreatine, a biochemical capacitor; 3) the sum of the coupled ATPases sets the demand side of the balance and defines energetic states; and 4) this demand is supplied by aerobic metabolism and the products of the coupled ATPases provide control signals for regulation of energy balance. We speculate that cytoplasmic signals at work in energy balance may also control muscle plasticity.


Human Skeletal Muscle Mechanical Power Output Phosphorus Nuclear Magnetic Resonance Muscle Glycogen Resynthesis ATPase Rate 
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  1. Arnold DL, Matthews PM & Radda GK (1984). Metabolic recovery after exercise and the assessment of mitochondrial function in vivo in human skeletal muscle by means of 31P NMR. Magnetic Resonance in Medicine 1, 307–315.PubMedCrossRefGoogle Scholar
  2. Blei ML, Conley KE, Odderson IR, Esselman PC & Kushmerick MJ (1993). Individual variation in contractile cost and recovery in human skeletal muscle. Proceeding of the National Academy of Science, USA 90, 7396–7400.CrossRefGoogle Scholar
  3. Bloch G, Chase JR, Meyer DB, Avison MJ, Shulman GI & Shulman RG (1994). In vivo regulation of rat muscle glycogen resynthesis after intense exercise. American Journal of Physiology (Endocrine Metabolism) 266, E85–E91.Google Scholar
  4. Bottinelli R, Schiaffino S & Reggiani C (1991). Force-velocity relations and myosin heavy chain isoform compositions of skinned fibres from rat skeletal muscle. Journal of Physiology (London) 437, 655–672.Google Scholar
  5. Brown GC (1992). Control of Respiration and ATP synthesis in mammalian mitochondria and cells. Biochemical Journal 284, 1–13.PubMedGoogle Scholar
  6. Chance B, Leigh J, Kent J, McCully K, Nioka S, Clark BJ & Maris JM (1986). Multiple controls of oxidative metabolism in living tissues as studied by phosphorus magnetic resonance. Proceedings of the National Academy of Science, USA 83, 9458–9462.CrossRefGoogle Scholar
  7. Conley KE (1994). Cellular energetics during exercise. Advances in Veterinary Science and Comparative Medicine 38A, 1–39.PubMedGoogle Scholar
  8. Connett RJ (1988). Analysis of metabolic control: new insights using scaled creatine kinase model. American Journal of Physiology 254, R949–R959.PubMedGoogle Scholar
  9. Crow MT & Kushmerick MJ (1982). Chemical energetics of slow-and fast-twitch muscles of the mouse. Journal of General Physiology 79, 147–166.PubMedCrossRefGoogle Scholar
  10. Das AM & Harris DA (1990). Regulation of the mitochondrial ATP synthase in intact rat cardiomyocytes. Biochemical Journal 266, 355–361.PubMedGoogle Scholar
  11. Edstrom L, Hultman E, Sahlin K & Sjoholm H (1982). The contents of high-energy phosphates in different fibre types in skeletal muscles from rat, guinea-pig, and man. Journal of Physiology (London) 332, 47–58.Google Scholar
  12. Firth JD, Ebert BL, Pugh CW & Ratcliffe PJ (1994). Oxygen-regulated control elements in the phosphoglycerate kinase 1 and lactate dehydrogenase A genes: Similarities with the erythropoietin 3’ enhancer. Proceedings of the National Academy of Science, USA 91, 6496–6500.CrossRefGoogle Scholar
  13. Goldberg MA, Dunning SP & Bunn HF (1988). Regulation of the erythropoietin gene: evidence that the oxygen sensor is a heme protein. Science 242, 1412–1414.PubMedCrossRefGoogle Scholar
  14. Gollnick PD, Armstrong RB, Sembrowich WL, Shepherd RE & Saltin B (1973). Glycogen depletion pattern in human skeletal muscle fibers after heavy exercise. Journal of Applied Physiology 34, 615–618.PubMedGoogle Scholar
  15. Greenhaff PL, Söderlund K, Ren JM & Hultman E (1993). Energy metabolism in single human muscle fibres during intermittent contraction with occluded circulation. Journal of Physiology (London) 460, 443–453.Google Scholar
  16. Heineman FW & Balaban RS (1990). Control of mitochondrial respiration in the heart in vivo. Annual Review of Physiology 52, 523–542.PubMedCrossRefGoogle Scholar
  17. Hintz, CS, Chi M M-Y, Fell RD, Ivy JL, Kaiser KK, Lowry CV & Lowry OH (1982). Metabolite changes in individual rat muscle fibers during stimulation. American Journal of Physiology 242, C218–C228.PubMedGoogle Scholar
  18. Hofmann PA, Hartzell HC & Moss RL (1991). Alterations in Ca2+ sensitive tension due to partial extraction of C-protein from rat skinned cardiac myocytes and rabbit skeletal muscle fibers. Journal of General Physiology 97, 1141–1163.PubMedCrossRefGoogle Scholar
  19. Kushmerick MJ (1977). Energy balance in muscle contraction: A biochemical approach. In: Sanadi R (ed.), Current Topics in Bioenergetics, vol. 6, pp. 1–15. New York: Academic Press.Google Scholar
  20. Kushmerick MJ (1983). Energetics of muscle contraction. In: Peachey L, Adrian R, Geiger SR (eds.), Handbook of Physiology, Skeletal Muscle, pp. 189–236. Bethesda: American Physiological Society.Google Scholar
  21. Kushmerick MJ & Davies RE (1969). The chemistry, efficiency and power of maximally working sartorius muscles. Proceedings of the Royal Society, London B, 315-353.Google Scholar
  22. Kushmerick MJ & Meyer RA (1985). Chemical changes in rat leg muscle by phosphorus nuclear magnetic resonance. American Journal of Physiology 248, C542–C549.PubMedGoogle Scholar
  23. Kushmerick MJ, Moerland TS & Wiseman RW (1992). Mammalian skeletal muscle fibers distinguished by contents of phosphocreatine, ATP, and Pi. Proceedings of the National Academy of Science, USA 89, 7521–7525.CrossRefGoogle Scholar
  24. Kushmerick MJ, Moerland TS & Wiseman RW (1993). Two classes of mammalian skeletal muscle fibers distinguished by metabolite content. Advances in Experimental Medicine and Biology 332, 749–761.PubMedCrossRefGoogle Scholar
  25. Kushmerick MJ & Paul RJ (1976). Aerobic recovery metabolism following a single isometric tetanus in frog sartorius muscle at 0°C. Journal of Physiology (London) 254, 693–709.Google Scholar
  26. Larsson L & Moss RL (1993). Maximum velocity of shortening in relation to myosin isoform composition in single fibres from human skeletal muscles. Journal of Physiology (London) 472, 595–614.Google Scholar
  27. Lipmann F (1941). Metabolic generation and utilization of phosphate bond energy. In: Nord FF, Werkman CH (eds.), Advances in Enzymology, vol 1, pp. 99–162. New York: Intersciences Publishers, Inc.Google Scholar
  28. McCormack JG & Denton RM (1990). Ca2+ as a second messenger within mitochondria in the heart and other tissues. Annual Review of Physiology 52, 451–466.PubMedCrossRefGoogle Scholar
  29. McCormack JG & Denton RM (1993). The role of intramitochondrial Ca2+ in the regulation of oxidative phosphorylation in mammalian tissues. Biochemical Society Transactions 21, 793–799.PubMedGoogle Scholar
  30. Metzger JM & Moss RL (1987). Shortening velocity in skinned single muscle fibers. Biophysical Journal 52, 127–8131.PubMedCrossRefGoogle Scholar
  31. Meyer RA (1989). Linear dependence of muscle phosphocreatine kinetics on total creatine content. American Journal of Physiology 257, C1149–C1157.PubMedGoogle Scholar
  32. Meyer RA, Brown TR, Krilowicz BL & Kushmerick MJ (1986). Phosphagen and intracellular pH changes during contraction of creatine-depleted rat muscle. American Journal of Physiology 250, C264–C274.PubMedGoogle Scholar
  33. Meyer RA, Brown TR, Kushmerick MJ (1985). Phosphorus nuclear magnetic resonance of fast-and slowtwitch muscle. American Journal of Physiology 248, C279–C287.PubMedGoogle Scholar
  34. Meyer RA, Dudley GA & Terjung RL (1980). Ammonia and IMP in different skeletal muscle fibers after exercise in rats. Journal of Applied Physiology 49, 1037–1041.PubMedGoogle Scholar
  35. Meyer RA, Sweeney HL & Kushmerick MJ (1984). A simple analysis of the “phosphocreatine shuttle.” American Journal of Physiology 246, C365–C377.PubMedGoogle Scholar
  36. Meyer RA & Terjung RL (1979). Differences in ammonia and adenylate metabolism in contracting fast and slow muscle. American Journal of Physiology 237, C111–C118.PubMedGoogle Scholar
  37. Mizuno M, Secher NH & Quistorff B (1994). 31P-NMR spectroscopy, rsEMG, and histochemical fiber types of human wrist flexor muscles. Journal of Applied Physiology 76, 531–538.PubMedGoogle Scholar
  38. Moerland TS, Wolf NG & Kushmerick MJ (1989). Administration of a creatine analogue induces isomyosin transitions in muscle. American Journal of Physiology 257, C810–C816.PubMedGoogle Scholar
  39. Pette D & Staron RS (1990). Cellular and molecular diversity of mammalian skeletal muscle fibers. Reviews of Physiology, Biochemistry & Pharmacology 116, 1–76.Google Scholar
  40. Pette D & Vrbova G (1992). Adaptation of mammalian skeletal muscle fibers to chronic electrical stimulation. Reviews of Physiology, Biochemistry & Pharmacology 120, 115–202.CrossRefGoogle Scholar
  41. Price TB, Rothman DL, Taylor R, Avison MJ, Shulman GI & Shulman RG (1994). Human muscle glycogen resynthesis after exercise: Insulin-dependent and-independent phases. Journal of Applied Physiology 76, 104–111.PubMedCrossRefGoogle Scholar
  42. Ren J-M & Hultman E (1989). Regulation of glycogenolysis in human skeletal muscle. Journal of Applied Physiology 67, 2243–2248.PubMedGoogle Scholar
  43. Saltin B & Gollnick PD (1983). Skeletal muscle adaptability: significance for metabolism and performance. In: Geiger SR, Adrian RH (eds.), Peachey LD (sec. ed.), Handbook of Physiology, sec. 10, Skeletal Muscle, pp. 555-631. Bethesda, MD: American Physiological Society.Google Scholar
  44. Shoubridge EA, Challiss RAJ, Hayes DJ & Radda GK (1985). Biochemical adaptation in the skeletal muscle of rats depleted of creatine with the substrate analogue B-guanidinopropionic acid. Biochemical Journal 232, 125–131.PubMedGoogle Scholar
  45. Söderlund K & Hultman E (1991). ATP and phosphocreatine changes in single human muscle fibers after intense electrical stimulation. American Journal of Physiology (Endocrine Metabolism) 261, E737–E741.Google Scholar
  46. Sweeney HL, Kushmerick MJ, Mabuchi K, Sreter FA & Gergely J (1988). Myosin alkali light chain and heavy chain variations correlate with altered shortening velocity of isolated skeletal muscle. Journal of Biological Chemistry 263, 9034–9039.PubMedGoogle Scholar
  47. Taylor DJ, Bore PJ, Styles P, Gadian DG & Radda GK (1983). Bioenergetics of intact human muscle. A 31P nuclear magnetic resonance study. Molecular Biology & Medicine 1, 77–94.Google Scholar
  48. Vandenborne K, McCully K, Kakihira H, Prammer M, Bolinger L, Detre JA, De Meirleir K, Walter G, Chance B & Leigh JS (1991). Metabolic heterogeneity in human calf muscle during maximal exercise. Proceedings of the National Academy of Science, USA 88, 5714–5718.CrossRefGoogle Scholar
  49. Vandenborne K, Walter G, Goelman G, Ploutz L, Dudley G & Leigh JS (1993a). Phosphate content of fast and slow twitch muscles. Proceedings of the Society of Magnetic Resonance in Medicine 3, 1140.Google Scholar
  50. Vandenbome K, Walter G, Leigh JS & Goelman G (1993b). pH heterogeneity during exercise in localized spectra from single human muscles. American Journal of Physiology (Cell Physiology) 265, C1332–C1339.Google Scholar
  51. Wallimann T, Wyss M, Brdiczka D, Nicolay K & Eppenberger HM (1992). Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands: the ‘phosphocreatine circuit’ for cellular energy homeostasis. Biochemical Journal 281, 21–40.PubMedGoogle Scholar
  52. Wilkie DR (1960). Thermodynamics and the interpretation of biological heat measurements. Progress in Biophysics and Biophysical Chemistry 10, 260–298.Google Scholar
  53. Woledge RC, Curtin NA & Homsher E (eds.) (1986). Energetic Aspects of Muscle Contraction. New York: Academic Press.Google Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • M. J. Kushmerick
    • 1
  1. 1.Departments of Radiology, Bioengineering, and PhysiologyUniversity of WashingtonSeattleUSA

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