Human Monocyte/Macrophage Serine Esterase-1: Expression in Haematopoiesis and Transcript Deficiency in Alveolar Macrophages Derived from Patients Affected by Pulmonary Fibrosis
Human monocytes and macrophages contain an esterolytic activity that hydrolyses alpha naphthyl acetate (ANA). The sensitivity of the monocyte esterase to the inhibitor diisopropylfluorophosphate classifies this enzyme as a carboxylesterase with a serine residue at the active site which is involved in hydrolysis. ANA cleaving enzymes are also present in other haematopoietic cells. Isoelectric focusing of proteins from purified haemapoietic cells reveals different patterns of ANA cleaving enzymes which are specific for each cell type. Monocytes contain at least five ANA cleaving enzymes with a predominant enzyme activity at pH 5.9 .
KeywordsHealthy Donor Alveolar Macrophage Idiopathic Pulmonary Fibrosis Pulmonary Fibrosis Peritoneal Macrophage
Unable to display preview. Download preview PDF.
- Radzen, H.J., Parwaresch, M.R., Kulenkampff, Ch., Staudinger, M., Stein, H.: Lysosomal acid esterase: Activity and isoenzymes in separated normal human blood cells. Blood 55 (1980), 891–897Google Scholar
- Becker-Follmann, J., Zschunke, F., Parwaresch, M.R., Radzun, H.J., Scherer, G.: Assignment of the human monocyte/macrophage serine esterase 1 (HMSE1) to human chromosome 16q13-q22.1 and of its homologue to the proximal esterase cluster on mouse chromosome 8. Cytogenet. Cell Genet. 58 (1991), 19–97.Google Scholar
- Takahashi K., Mishima, K., Ichikawa, Y., Watanabe, K., Komatsuda, M., Arimori, S.,: Report of a case with chronic myelomonocytic monocyte esterase deficiency. Tokai J. Exp. and Clin. Med. 12 (1987), 275–281Google Scholar
- Heymann, E, In: Enzymatic Basis of Detoxication, ed. Jakoby, W.P. Academic Press, New York (1980) 291–323.Google Scholar