Advertisement

Human Monocyte/Macrophage Serine Esterase-1: Expression in Haematopoiesis and Transcript Deficiency in Alveolar Macrophages Derived from Patients Affected by Pulmonary Fibrosis

  • F. Zschunke
  • J. Barth
  • H. Kreipe
  • H. J. Radzun
Chapter
Part of the NATO ASI Series book series (NSSA, volume 266)

Abstract

Human monocytes and macrophages contain an esterolytic activity that hydrolyses alpha naphthyl acetate (ANA). The sensitivity of the monocyte esterase to the inhibitor diisopropylfluorophosphate classifies this enzyme as a carboxylesterase with a serine residue at the active site which is involved in hydrolysis. ANA cleaving enzymes are also present in other haematopoietic cells. Isoelectric focusing of proteins from purified haemapoietic cells reveals different patterns of ANA cleaving enzymes which are specific for each cell type. Monocytes contain at least five ANA cleaving enzymes with a predominant enzyme activity at pH 5.9 [1].

Keywords

Healthy Donor Alveolar Macrophage Idiopathic Pulmonary Fibrosis Pulmonary Fibrosis Peritoneal Macrophage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Radzen, H.J., Parwaresch, M.R., Kulenkampff, Ch., Staudinger, M., Stein, H.: Lysosomal acid esterase: Activity and isoenzymes in separated normal human blood cells. Blood 55 (1980), 891–897Google Scholar
  2. [2]
    Zschunke, F., Salmassi, A., Kreipe, H., Parwaresch, M.R., Radzun, H.J.: cDNA cloning and characterization of Human Monocyte/Macrophage Serine Esterase-1. Blood, 78 (1991), 506–512.PubMedGoogle Scholar
  3. [3]
    Zschunke, F., Salmassi, A., Kreipe, H., Parwaresch, M.R., Radzun, H.J.: Heterogenous expression and putative structure of human monocyte/macrophage serine esterase 1. Res. Immunol. 143 (1992), 125–128.PubMedCrossRefGoogle Scholar
  4. [4]
    Uphoff, C.C., Gignac, S.M., Metge, K., Zschunke, F., Radzun, H.J., Drexler, H.G.: Expression of the monocyte-specific Esterase gene in leukemia-lymphoma cell lines. Leukemia 7 (1993), 58–62.PubMedGoogle Scholar
  5. [5]
    Becker-Follmann, J., Zschunke, F., Parwaresch, M.R., Radzun, H.J., Scherer, G.: Assignment of the human monocyte/macrophage serine esterase 1 (HMSE1) to human chromosome 16q13-q22.1 and of its homologue to the proximal esterase cluster on mouse chromosome 8. Cytogenet. Cell Genet. 58 (1991), 19–97.Google Scholar
  6. [6]
    Boyum, A.: Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Clin. Lab. Invest. 21 (1968), 77–109CrossRefGoogle Scholar
  7. [7]
    Bennett, W.E., Cohn, Z.A.: The isolation and selected properties of blood monocytes. J. Exp. Med. 123 (1966), 145–157PubMedCrossRefGoogle Scholar
  8. [8]
    Markey, G.M., McCormick, J.A., Moms, T.C.M., Alexander, H.D., Nolan, L., Morgan, L.M., Reynolds, M.E., Edgar, S., Bell, A.L., McCaigue, M.D., Robertson, J.H.: Monocyte esterase deficiency in malignant neoplasia. J. Clin. Pathol. 45 (1990), 282–286CrossRefGoogle Scholar
  9. [9]
    Takahashi K., Mishima, K., Ichikawa, Y., Watanabe, K., Komatsuda, M., Arimori, S.,: Report of a case with chronic myelomonocytic monocyte esterase deficiency. Tokai J. Exp. and Clin. Med. 12 (1987), 275–281Google Scholar
  10. [10]
    Heymann, E, In: Enzymatic Basis of Detoxication, ed. Jakoby, W.P. Academic Press, New York (1980) 291–323.Google Scholar
  11. [11]
    Le Beau M.M., Diaz, M.O., Karin, M., Rowley, J.D.: Metallothionein gene cluster is split by chromosome 16 rearrangements in myelomonocytic leukemia. Nature 313 (1985), 709–711PubMedCrossRefGoogle Scholar
  12. [12]
    Saboori, A.M., Newcombe, D.S.: Human Monocyte Carboxylesterase. J. Biol. Chem. 265 (1990), 19792–19799PubMedGoogle Scholar
  13. [13]
    Salmassi, A., Kreipe, H., Radzun, H.J., Lilischkis, R., Charchinajad-Amoey, M., Zschunke, F., Buck, F., Lottspeich, F., Parwaresch, M.R.: Isolation of monocytic serine esterase and evaluation of its proteolytic activity. J. Leukocyte Biol. 51 (1992), 409–414PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • F. Zschunke
    • 1
  • J. Barth
    • 2
  • H. Kreipe
    • 3
  • H. J. Radzun
    • 1
  1. 1.Center of PathologyGeorg-August University GöttingenGöttingenGermany
  2. 2.Int. Medicine IChristian-Albrecht University KielKielGermany
  3. 3.Inst. for PathologyChristian-Albrecht University KielKielGermany

Personalised recommendations