Methods for the Determination of the Activity of Phospholipases and their Clinical Application

E. coli-based assay compared to a photometric micelle assay
  • Johannes Aufenanger
  • Michael Samman
  • Michael Quintel
Part of the NATO ASI Series book series (NSSA, volume 266)


Many assays for the determination of phospholipase A2 activity [for review: 1] have been developed. The choice of a detection method depends partly on the purpose of a particular experiment. For example, some assays can be used on purified enzymes but are incompatible with crude systems, some methods provide a continuous assay and generate a time course while others do not, and some methods are amenable to automation while others are not. However, the most important consideration in the choice of the detection method is the sensitivity required for the particular enzyme which depends on the quantity of enzyme available and on its specific activity. This point is especially important for the assay of phospholipases A2 in human plasma, which are found in lower quantities and are, in general, less active than their counterparts from venom.


Acute Pancreatitis Pancreatic Lipase Fatty Acid Concentration Free Fatty Acid Concentration Label Fatty Acid 
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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Johannes Aufenanger
    • 1
  • Michael Samman
    • 1
  • Michael Quintel
    • 2
  1. 1.Institute for Clinical ChemistryFaculty for Clinical Medicine of the University of HeidelbergMannheimGermany
  2. 2.Institute for Clinical Anaesthesiology and Surgical Intensive Care Medicine Klinikum MannheimFaculty for Clinical Medicine of the University of HeidelbergMannheimGermany

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