Rabbit Pancreatic Lipase: Purification and Some Novel Catalytic Properties
One of the main features of lipolysis is that it occurs as a two-step reaction. In the first step, triglycerides (TG) are hydrolyzed producing 1,2diglycerides (DG) which are subsequently hydrolyzed to 2-monoglycerides (MG). Therefore, DG constitute both the first product of lipolysis and the substrate for the second reaction. In order to compare these reactions, lipolysis has been considered to be a common chemical sequential reaction. Following the determination of the relative concentrations of the component parts, the rate constants of the two reactions could be calculated. These rate constants are the kinetic parameters we use in order to compare the two sequential reactions.
KeywordsZeta Potential Bile Salt Lipase Activity Mono Glyceride Mixed Micelle
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- 3.P. Arzoglou, A. Tavridou, JM. Lessinger, G. Tzimas, and G. Férard, Spectro photometric determination of lipase activity in the presence of increased triolein concentration, Ann. Biol. Clin. 50:(1992)155–160Google Scholar