Rabbit Pancreatic Lipase: Purification and Some Novel Catalytic Properties

  • A. Lykidis
  • A. Avranas
  • V. Mougios
  • P. Arzoglou
Chapter
Part of the NATO ASI Series book series (NSSA, volume 266)

Abstract

One of the main features of lipolysis is that it occurs as a two-step reaction. In the first step, triglycerides (TG) are hydrolyzed producing 1,2diglycerides (DG) which are subsequently hydrolyzed to 2-monoglycerides (MG). Therefore, DG constitute both the first product of lipolysis and the substrate for the second reaction. In order to compare these reactions, lipolysis has been considered to be a common chemical sequential reaction. Following the determination of the relative concentrations of the component parts, the rate constants of the two reactions could be calculated. These rate constants are the kinetic parameters we use in order to compare the two sequential reactions.

Keywords

Zeta Potential Bile Salt Lipase Activity Mono Glyceride Mixed Micelle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. Tavridou, A. Avranas, and P. Arzoglou, A mathematical approach to lipolysis based on the interrelationship of physicochemical and biochemical data, Biochem. Biophys. Res. Commun. 186: (1992) 746–752PubMedCrossRefGoogle Scholar
  2. 2.
    D. Lairon, G. Nalbone, H. Lafont, J. Leonardi, N. Domingo, J.C. Hauton, R. Verger, Possible roles of bile lipids and colipase in lipase adsorption, Biochemistry. 17. (1978) 5263–5269PubMedCrossRefGoogle Scholar
  3. 3.
    P. Arzoglou, A. Tavridou, JM. Lessinger, G. Tzimas, and G. Férard, Spectro photometric determination of lipase activity in the presence of increased triolein concentration, Ann. Biol. Clin. 50:(1992)155–160Google Scholar
  4. 4.
    A. Avranas, G. Stalidis, and G. Ritzoulis, Demulsification rate and zeta potential of 01W emulsions, Colloid Polym. Sci. 266: (1988) 937–940CrossRefGoogle Scholar
  5. 5.
    N. Mazer, G. Benedek, and M. Carey, Quasielastic light-scattering studies of aqueous biliary lipid systems. Mixed micelle formation in bile salt-lecithin solutions, Biochemistry. 19: (1980) 601–615PubMedCrossRefGoogle Scholar
  6. 6.
    C. Erlanson-Albertsson, The interaction between pancreatic lipase and colipase: a protein-protein interaction regulated by a lipid, FEBS Lett. 162: (1983) 225–229PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • A. Lykidis
    • 1
  • A. Avranas
    • 2
  • V. Mougios
    • 3
  • P. Arzoglou
    • 1
  1. 1.Laboratory of Biochemistry, School of ChemistryAristotle University of ThessalonikiThessalonikiGreece
  2. 2.Laboratory of Physical Chemistry, School of ChemistryAristotle University of ThessalonikiThessalonikiGreece
  3. 3.Laboratory of Exercise Biochemistry, Department of Physical Education and Sport ScienceAristotle University of ThessalonikiThessalonikiGreece

Personalised recommendations