Modern Approaches to the Taxonomy of Aspergillus
Techniques from molecular biology have provided a series of new tools for the analysis of diversity in the fungi. These techniques have been applied to a variety of fungal groups but only rather limited work has been done on the genus Aspergillus, which is surprising considering the economic importance of species within the genus. The availability of a detailed molecular genetic systems in Aspergillus nidulans has been of considerable help in providing a scientific base, but it appears on the whole that molecular geneticists have not been very interested in the taxonomy of the genus. However, a need to study the epidemiology, detection, diagnosis, identification, classification, characterisation and quantification of Aspergillus has resulted in an increasing interest in the taxonomic basis for differences within the genus. This has made it essential that closer links are forged between molecular biologists and taxonomists.
KeywordsInternally Transcribe Spacer Aspergillus Nidulans Slot Blot Nucleic Acid Probe External Transcribe Spacer
Unable to display preview. Download preview PDF.
- Aufauvre-Brown, A., Cohen, J. and Holden, D.W. (1992) Use of randomly amplified polymorphic DNA markers to distinguish isolates of Aspergillus fumigatus. J. Clinical Micro. 30, 2991–2993.Google Scholar
- Bainbridge, B.W., Spreadbury, C.L., Scalise, E.G. and Cohen, J. (1990) Improved methods for the preparation of high molecular weight DNA from large and small scale cultures of filamentous fungi. FEMS Micro. Lett. 66, 113–118.Google Scholar
- Eckert, K.A. and Kunkel, T.A. (1991) The fidelity of DNA polymerases used in the polymerase chain reactions in “PCR a practical approach” (McPherson, M.J., Quirke, P. and Taylor, G.R. Eds.) pp 225–244. IRL Press, Oxford. Edman, J.C.Google Scholar
- Goodwin, P.H. and Annis, S.L. (1991) Rapid identification of Genetic Variation and pathotype of Leptosphaeria maculans by Random Amplified Polymorphic DNA assay. Appl. Environ. Micro. 57, 2482–2486.Google Scholar
- Jefferys, A.J., MacLeod, A., Tanaki, K., Neil, D.L. and Monckton, D.G. (1991) Minisatellite repeat coding as a digital approach to DNA typing. Nature, 354, 201–209.Google Scholar
- Klich, M.A. and Pitt, J.I. (1988) A laboratory guide to common Aspergillus species and their teleomorphs. Commonwealth Scientific and Industrial Research Organisation, North Ryde, New South Wales, Australia.Google Scholar
- Moens, W. (1992) Fast design of Fungal PCR Markers. Proceedings of the Ist European Conference on Fungal Genetics at Nottingham August 1992 P2/58.Google Scholar
- Payne, G.A., Nystrom, G.J., Bhatnagar, D. Cleveland, T.E. and Woloshuk, C.P. (1993) Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus. Appl. Environ. Micro. 59, 156–162.Google Scholar
- Sober, E. (1989) Reconstructing the past: Parsimony, evolution, and inference. M.T Press, Cambridge, MA, USA.Google Scholar
- Spreadbury, C.X., Bainbridge, B.W. and Cohen, J. (1990) Restriction fragment length polymorphisms in isolates of Aspergillus fumigatus probed with part of the intergenic spacer region from the ribosomal RNA gene complex of Aspergillus nidulans . J. Gen. Microbiol. 136, 1991–1994.PubMedCrossRefGoogle Scholar
- Swofford, D.L. and Olsen, G.J. (1990) Phylogeny reconstruction in “Molecular Systematics” (Hillis, D.M. and Moritz, C., Eds.) pp. 411–501. Sinhauer, Sunderland, Mass, U.S.A.Google Scholar
- White, T.J., Bruns, T., Lee, S. and Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, in “PCR Protocols: a Guide to Methods and Applications” (Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J. Eds.) pp. 315–322. Acad. Press, Inc. San Diego.Google Scholar