Molecular Genetics of the bimB and bimD Genes of Aspergillus Nidulans, Two Genes Required for Mitosis
Advances in understanding how cells duplicate and segregate their constituents through the cell cycle have been greatly facilitated by the genetic analysis of these processes in microbial eukaryotic organisms. The most notable of these organisms are the yeasts Saccaharomyces cerevisiae and Schizosaccharomyces pombe and the filamentous fungus Aspergillus nidulans. Much of this work has been directed towards understanding the regulators of major transition points in the cell cycle such as the transitions from Gl to S-phase and G2 to mitosis. For reviews of this literature the reader is referred to the following references: Doonan; 1992; Hartwell and Weinert, 1989; Murray, 1992. The advances made in A. nidulans were made possible by the isolation of a collection of heat sensitive (hs) mutants (Morris, 1976). This collection of hs mutants contained a class of mutations that altered the cell’s ability to traverse the cell cycle at restrictive temperature and in some cases resulted in a block in mitosis. Those mutations that resulted in a block in mitosis were designated bim and it is the bim genes that will be discussed here in some detail.
KeywordsAspergillus Nidulans Transcriptional Unit Restrictive Temperature Permissive Temperature Kinetochore Microtubule
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