Transcriptional Silencer in Intron I of the Rat Renin Gene

  • T. Voigtländer
  • A. Ripperger
  • D. Ganten
  • M. Bader
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 377)


In the present study we investigated the influence of intron I of the rat renin gene on the transcriptional activity of its promoter in cell culture. The presence of intron I abolished the transcription of reporter genes (luciferase and lacZ) in the non-renin-expressing human embryonic kidney cell line 293, while it did not significantly affect the activity of the rat renin promoter in rat sceletal myoblast line L8 expressing renin. We conclude from these results that intron I of the rat renin gene contains a tissue-specific silencer element probably also responsible for the transcriptional repression of the endogeneous renin gene in 293 cells.


Human Embryonic Kidney Cell Human Embryonic Kidney Cell Line Renin Gene Injection Chamber lacZ Activity 
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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • T. Voigtländer
    • 1
  • A. Ripperger
    • 1
  • D. Ganten
    • 1
  • M. Bader
    • 1
  1. 1.Max-Delbrück-Center for Molecular Medicine (MDC)Hypertension ResearchBerlin-BuchGermany

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