Advertisement

Reversibility of Changes in Rat Liver DNA Methylation and Gene Expression Induced by a Methyl-Deficient Diet

  • J. K. Christman
  • G. Sheikhnejad
  • M. Dizik
  • S. Abileah
  • E. Wainfan
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 375)

Abstract

As we have previously reported, both t-RNA and DNA become rapidly hypomethylated in livers of rats fed a cancer promoting, methyl-deficient diet [MDD-an amino acid defined, semisynthetic diet (AAD) devoid of choline, methionine, folate and vitamin B12 and supplemented with 9 g/kg D, L-homocystine]. Hypomethylation is detectable within one week and increases over a four week period of continued feeding of MDD.1 Hypomethylation of specific CCGG sites is observed within several genes for which mRNA levels are increased (c-myc, c -fos and c-Ha-ras).2 Interestingly, even though methylation of both DNA and tRNA is inhibited in the absence of a dietary source of methyl groups, the activity of DNA and tRNA MTases increases 2–3 fold.3

Keywords

Dietary Source Choline Chloride Continue Feeding Rapid Appearance Cancer Foundation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • J. K. Christman
    • 1
  • G. Sheikhnejad
    • 1
  • M. Dizik
    • 1
  • S. Abileah
    • 1
  • E. Wainfan
    • 1
  1. 1.Michigan Cancer Foundation and the Meyer L. Prentis Comprehensive Cancer Center of Metropolitan DetroitDetroitUSA

Personalised recommendations