Efficacy of Pyridoxal Therapy in Controlling the Growth of Melanomas in Cell Culture and an Animal Pilot Study

Abstract

We have demonstrated, using confocal laser scanning microscopy, that pyridoxal treatment of B16C3 murine melanoma cells inhibits triamcinolone acetonide induced translocation of the glucocorticoid receptor (GR) to the nucleus. Further investigations extended our work with murine cell cultures, demonstrating that pyridoxal also kills WM9-83A human melanoma cells in culture. In studies with glucocorticoid antagonists, cortexolone functions to inhibit cell proliferation. This mechanism, however, appears to initiate in the GR signal transducing cascade at a point prior to the impact of pyridoxal treatment alone. RU486 alone has no detrimental effect on melanoma cell viability. However, in combination with pyridoxal, RU486 extends cell viability.