Abstract
Recent advances in laboratory diagnostic technology has shifted from classical procedures such as serum neutralization and complement fixation, to procedures that are more rapid, specific, sensitive and generally less expensive. Accordingly for routine serologic diagnosis in the virus laboratory, ELISA-EIA has replaced for practical purposes, those first generation procedures. Here too, modifications of the original procedure have resulted in simplified methodologies that have practically eliminated the need for laboratory equipment. Development in this laboratory of a dot-immunobinding assay (DIA) for the rapid detection of viral antibody is one such method. Application of this DIA to a variety of different viral antigens has clearly demonstrated its usefulness in the field or office setting. One area of the test requiring some laboratory support was that of using patients’ serum which necessitated use of a syringe and generally a centrifuge. That whole blood could be used rather than serum and, further, applied directly onto the DIA test system was recently demonstrated. Blood collected on filter paper precut to fit the test following a finger stick has been successfully applied to the DIA. Further, blood collected in this fashion may be shipped via the mail and remains stable for relatively long periods of time. DIA results using whole blood or serum were identical. No increased nonspecific reactions or interfering background was observed.
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Authors and Affiliations
Southwest Foundation for Biomedical Research, NIH and WHO Collaborating Center for Reference and Research in Simian Viruses, San Antonio, Texas, USA
S. S. Kalter & R. L. Heberling
Syva Company, Palo Alto, California, USA
M. Banach & B. Judson
Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
C. Gleaves
Monoclonal Antibodies, Inc., Mountain View, California, USA
C. Fraser, R. Kamibayashi, M. Kawula, M. Hay-Kaufman & R. Fraser
Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
D. A. Hursh, S. F. Wendt, J. D. Meyers & C. A. Gleaves
University of Utah School of Medicine, Salt Lake City, Utah, USA
W. E. Taggart, B. R. Kenyon, V. C. Salmon & J. C. Overall
Viral Laboratory, Laboratory Services, San Jose, California, USA
E. L. Sheldon & D. Andresen
University of California Irvine Medical Center, Orange, California, USA
S. L. Aarnaes, L. M. de la Maza & E. M. Peterson
Steller Bio Systems, Inc., Columbia, Maryland, USA
F. K. Mundon, J. M. Brewer, P. P. Brewer & W. E. Barton
New England Deaconess Hospital, Boston, Massachusetts, USA
P. C. de Girolami, J. Dakos, J. Schiff, S. Bolivar & L. Merrill
Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
C. A. Gleaves, S. E. Curtis, C. F. Lee & J. D. Meyers
Mayo Clinic and Foundation, Rochester, Minnesota, USA
C. V. Paya, A. D. Wold & T. F. Smith
Children’s Hospital Medical Center, University of Washington, Seattle, Washington, USA
C. Cozza, H. Abbo, M. Allen & R. Ashley
The Ohio State University and Children’s Hospital, Columbus, Ohio, USA
M. T. Brady & J. F. Cuartas
Diskind Pathology Research Laboratory, Mount Zion Hospital and Medical Center, San Francisco, California, USA
R. C. Miner, N. Rabella & W. L. Drew
Viral and Rickettsial Disease Laboratory, California State Department of Health Services, Berkeley, California, USA
B. Forghani, G-J. Yu & J. W. Hurst
Bellin Memorial Hospital, Green Bay, Wisconsin, USA
C. Siegel & S. Goodreau
San Diego School of Medicine, University of California, La Jolla, California, USA
P. Walpita & S. Darougar
Institute of Ophthalmology, London, England
P. Walpita & S. Darougar
Cambridge BioScience Corp, Worcester, Massachusetts, USA
D. Leombruno
Smith Kline BioScience, Van Nuys, California, USA
M. J. August
Kings County Hospital, Brooklyn, New Jersey, USA
K. Bromberg
Children’s Hospital, Boston, Massachusetts, USA
L. T. Pierik
Cleveland Clinic Foundation, Cleveland, Ohio, USA
M. Bahn, A. Roberto, B. Yen-Lieberman & M. R. Proffitt
Mayo Clinic and Mayo Foundation, Rochester, Minnesota, USA
M. J. Espy, E. J. Jespersen & T. F. Smith
Tunney’s Pasture, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada
D. A. Kennedy, R. A. Hills, D. Dick, M. T. Monette & G. Butler
Graduate School of Public Health, San Diego State University, San Diego, California, USA
K. M. Peddecord, A. S. Beneson, L. K. Hofherr, M. S. Ascher & R. Elwell
California Viral and Rickettsial Laboratories, Berkeley, California, USA
K. M. Peddecord, A. S. Beneson, L. K. Hofherr, M. S. Ascher & R. Elwell
Centers for Disease Control, Atlanta, Georgia, USA
K. M. Peddecord, A. S. Beneson, L. K. Hofherr, M. S. Ascher & R. Elwell
Triton Biosciences, Inc., Alameda, California, USA
D. Parkes, C. M. Smith, J. Brandis & S. R. Coates
Triton Biosciences, Inc., Alameda, California, USA
A. J. Harris, M. Ferrer, C. M. Smith, D. di Ditullio, J. Brandis, M. Sliwkowski & S. Coates
Triton Biosciences, Inc., Alameda, California, USA
C. M. Christian, C. M. Smith, J. Brandis & S. Coates
Triton Biosciences, Inc., Alameda, California, USA
E. L. Keitelman, S. A. Wallingford, R. Akita, C. M. Smith, M. Ferrer, R. Akita, A. Harris, H.-L. Liu, M. X. Sliwkowski, M. W. Brandis & Y. A. Teramoto
Triton Biosciences, Inc., Alameda, California, USA
B. C. Langton, M. Sliwkowski, K. V. Tran, S. Knapp, E. Keitelman, C. Smith, S. Wallingford, H.-L. Liu, J. S. Ralston, J. Brandis & S. Coates
Organon Teknika Corp., Durham, North Carolina, USA
C. Chetty, J. W. Northing, P. D. Scruggs, P. L. Tan, T. S. Flowers & J. W. D. Kay
University of Texas Medical Branch, Galveston, Texas, USA
R. R. McKendall & W. Woo
University of Liverpool, Liverpool, England
A. M. Al-Sumidate & J. C. Woodrow
Children’s Hospital, Columbus, Ohio, USA
M. T. Brady & J. F. Cuartas
Veterans Administration Medical Center, University of California, San Francisco, San Francisco, California, USA
L. Pulliam, N. Tang & P. Simpson
The Richmond City Health Department, The Medical College of Virginia, Richmond, Virginia, USA
R. J. Rice, M. Neff-Smith & F. E. Churchill
The Richmond City Health Department, The Medical College of Virginia, Richmond, Virginia, USA
M. Neff-Smith & R. J. Rice
Dallas-Fort Worth Medical Center Research Laboratory, Grand Prairie, Texas, USA
H. R. McDaniel, T. Pulse, B. H. McAnalley & R. H. Carpenter
The Johns Hopkins University Oncology Center, Baltimore, Maryland, USA
D. P. Bednarik, J. D. Mosca, N. B. K. Raj & P. M. Pitha
Ohio Wesleyan University, Delaware, Ohio, USA
G. Goldstein
Mayo Clinic and Mayo Foundation, Rochester, Minnesota, USA
A. Telenti & T. F. Smith
Doheny Eye Institute, Los Angeles, California, USA
M. D. Trousdale & W. E. Barlow
Ciba-Geigy Corp., Summit, New Jersey, USA
L. J. B. McGuigan
Virginia Tech, College of Veterinary Medicine, Blacksburg, Virginia, USA
T. E. Toth & R. E. Hand
Oakridge National Laboratory, Oakridge, Tennessee, USA
T. E. Toth & R. E. Hand
Triton Biosciences, Inc., Alameda, California, USA
D. Larocca, L. A. Chao, M. Wright, C. Smith & S. Coates
Kanazawa Medical University, Uchinada, Ishikawa, Japan
X. Bin, T. Murayama, K. Ishida & T. Furukawa
University of Colorado Health Sciences Center, Denver, Colorado, USA
R. W. Farr, P. Bishop & A. Siddiqui
Los Angeles School of Medicine, University of California, Los Angeles, California, USA
R. Gaynor
Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada
A. Cepica, C. Yason & G. Ralling
School of Medicine and Norris Hospital and Research Institute, University of Southern California, Los Angeles, California, USA
R. Agah, P. E. Twomey, L. Buffa & A. Mazumder
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Kalter, S.S. et al. (1989). Abstracts. In: de la Maza, L.M., Peterson, E.M. (eds) Medical Virology 8. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0891-9_14
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