Urolithiasis pp 239-240 | Cite as

Adherence of Glycosaminoglycans to Calcium-Oxalate Crystals

  • A. H. Angell
  • M. I. Resnick

Abstract

Calcium oxalate (CaOx) crystals were manufactured in a 10-mM Ca++ excess solution by combining 5.0 ml of 0.3-M K-oxalate, 1.0 ml of 1.65-M CaCl2, and 1.0 ml of 1.5-M Na-cacodylate buffer (pH 7.3, 20°C) in borosilicate glass vials; the mixture was agitated for 30 min in a thermostatically-controlled shaker bath (37°C). The macromolecules were previously dissolved in 0.3-M KC1. The mixture was placed back in the water bath for 2 h, centrifuged at 200 g for five min, and the supernatant was filtered through 0.2-μm polycarbonate filters. The equilibrium concentration of the adsorbate was determined by measuring the concentration of glycosaminoglycan (GAG) (uronic acid) using the solution-depletion method.

Keywords

Hyaluronic Acid Heparan Sulfate Chondroitin Sulfate Calcium Oxalate Dermatan Sulfate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • A. H. Angell
    • 1
  • M. I. Resnick
    • 1
  1. 1.Division of UrologyCase Western Reserve University School of MedicineClevelandUSA

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