Urolithiasis pp 121-123 | Cite as

Membrane-Induced Calcium-Oxalate Crystal Nucleation

  • S. R. Khan
  • R. L. Hackett


Calcium oxalate (CaOx) crystals experimentally induced in rat kidneys as well as those found in human kidneys are almost always associated with PAS and colloidal iron-positive material (1). Transmission electron microscopy of the crystalline deposits in rat renal tubules revealed the associated material to consist of amorphous as well as membranous cellular degradation products (MCDP). Since CaOx relative supersaturation (RSS) of both stoneforming rat as well as human urine is much less than required for homogeneous nucleation of CaOx, it is suggested that crystal nucleation in urolithiasis is heterogeneous (2). Since the CaOx-crystal MCDP association appears universal, we decided to investigate, in vitro, the possibility of membranes playing a role in crystal nucleation. We used renal brush border membrane (BBM) because renal proximal tubules have been implicated in urolithiasis (3).


Calcium Oxalate Brush Border Membrane Scanning Electron Micro Renal Proximal Tubule Crystal Nucleation 
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  1. 1.
    SR Khan and RL Hackett, Experimental calcium oxalate nephrolithiasis, is it a model for human stone disease? Scanning Eleeton Micros. II: 759 (1985).Google Scholar
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    B Finlayson, Physico-chemical aspects of urolithiasis, Kidney Internat. 13: 344 (1978).CrossRefGoogle Scholar
  3. 3.
    RS Malek and WH Boyce, Intranephronic calculosis: its significance and relationship to matrix in nephrolithiasis, J. Urol. 109: 5551 (1973).Google Scholar
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    PG Werness, CM Brown, LH Smith, and B Finlayson, Equil 2: a BASIC computer program for the calculation of urinary saturation, J. Urol. 134: 1242 (1985).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • S. R. Khan
    • 1
  • R. L. Hackett
    • 1
  1. 1.Department of Pathology, College of MedicineUniversity of FloridaGainesvilleUSA

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