Membrane-Induced Calcium-Oxalate Crystal Nucleation
Calcium oxalate (CaOx) crystals experimentally induced in rat kidneys as well as those found in human kidneys are almost always associated with PAS and colloidal iron-positive material (1). Transmission electron microscopy of the crystalline deposits in rat renal tubules revealed the associated material to consist of amorphous as well as membranous cellular degradation products (MCDP). Since CaOx relative supersaturation (RSS) of both stoneforming rat as well as human urine is much less than required for homogeneous nucleation of CaOx, it is suggested that crystal nucleation in urolithiasis is heterogeneous (2). Since the CaOx-crystal MCDP association appears universal, we decided to investigate, in vitro, the possibility of membranes playing a role in crystal nucleation. We used renal brush border membrane (BBM) because renal proximal tubules have been implicated in urolithiasis (3).
KeywordsCalcium Oxalate Brush Border Membrane Scanning Electron Micro Renal Proximal Tubule Crystal Nucleation
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